QUESTION 1 You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0.158 1 0.210 0.260 3 0.305 4 0.360 0.410 6 0.455 7 0.510 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
QUESTION 1 You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0.158 1 0.210 0.260 3 0.305 4 0.360 0.410 6 0.455 7 0.510 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![QUESTION 1
You obtained the following raw data when setting up a Biuret standard curve:
BSA (mg/ml)
Absorbancy
540nm
0.158
1
0.210
2.
0.260
3
0.305
4
0.360
0.410
0.455
7
0.510
8
0.530
0.550
10
0.554
After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was
obtained
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant
figures).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F92756ff9-90e2-4c3c-90cf-dce0881ff676%2Fcad9cba1-ad55-44e4-93a5-8ff59d46155b%2Foiop49r_processed.png&w=3840&q=75)
Transcribed Image Text:QUESTION 1
You obtained the following raw data when setting up a Biuret standard curve:
BSA (mg/ml)
Absorbancy
540nm
0.158
1
0.210
2.
0.260
3
0.305
4
0.360
0.410
0.455
7
0.510
8
0.530
0.550
10
0.554
After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was
obtained
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant
figures).
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