What is the concentration of a solution with an absorbance at 520 nm (A520) of 0.6 that has an extinction coefficient of 3.0 L/(mmol cm) (assume pathlength is 1 cm and provide your answer should in units of mM, but do NOT write the mM).

What is the concentration of a solution with an absorbance at 520 nm (A520) of 0.6 that has an extinction coefficient of 3.0 L/(mmol cm) (assume pathlength is 1 cm and provide your answer should in units of mM, but do NOT write the mM).

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

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Iam stuck on how to start this problem. Youo are presented with a sample of freshly squeezed carrot juice and are asked to determine how much beta-carotene is presented in the juice. A beer's Law plots is constructed from the absorbances at 451 nm for six standard solutions of beta-carotene, with concentrations ranging from 0.75-7.5 µM. Linear regressions data: y=0.1431x-0.0078(R2=0.9998) a)The carrot juice is diluted by one-fourth, and the absorbance at 451 nm of this solutions is measured to be 0.676. Based on this information, what is molor concentration of beta-corotene in the original(undilited) sample of carrot juice? b) If the original sample of carrot juice was 312 mL, calculated the mass(g) of beta-carotene presented
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Can you help me on questions 2 AND 3
Hh.130.
2. ] You make 150.0 mL of a copper(II) sulfate solution but forget to cover it before leaving for the day. The next class, you measure the volume and find that you only have 132.0 mL remaining. You didn’t record the initial concentration, but you measure the absorbance of the remaining solution at 620 nm and find that it is 0.386. You also construct the following calibration curve for CuSO4: Standard solution Abs620 0.50 M 0.424 0.40 M 0.336 0.30 M 0.247 0.20 M 0.159 (refer to image) a. What is the value for k (in Beer’s Law)? Provide a calculation or graph to support your answer. b. What is the concentration of the (remaining) solution? c. What was the concentration of the original solution from the first class? d. If you removed 7.5 mL from the 132.0 mL solution in the process of measuring the absorbance, how much water should you add to obtain the original concentration that you solved for in part (c)?
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