A stock solution of Methylene Blue dye has absorbance (A) of 1.692 at 668 nm. How many fold (times) do you need to dilute this solution to obtain a working solution having absorbance of 0.150? How many microliters (ul) of the stock solution will be necessary to prepare 3.5 ml of the working solution? 668/1.692 394.8 fold; 3.5 ml / 394.8 8.87 x 10^-3 ml = 8.87 ul 3.5 ml / 0.150 = 23.3 fold; 1.692 x 23.3 = 39.4 ul 1.692/0.150 = 11.28 fold; 3.5 ml / 11.28 = 0.310 ml = 310 ul
A stock solution of Methylene Blue dye has absorbance (A) of 1.692 at 668 nm. How many fold (times) do you need to dilute this solution to obtain a working solution having absorbance of 0.150? How many microliters (ul) of the stock solution will be necessary to prepare 3.5 ml of the working solution? 668/1.692 394.8 fold; 3.5 ml / 394.8 8.87 x 10^-3 ml = 8.87 ul 3.5 ml / 0.150 = 23.3 fold; 1.692 x 23.3 = 39.4 ul 1.692/0.150 = 11.28 fold; 3.5 ml / 11.28 = 0.310 ml = 310 ul
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![Question 6
A stock solution of Methylene Blue dye has absorbance (A) of 1.692 at 668 nm. How many fold (times) do you
need to dilute this solution to obtain a working solution having absorbance of 0.150? How many microliters (ul)
of the stock solution will be necessary to prepare 3.5 ml of the working solution?
3
668/ 1.692 394.8 fold; 3.5 ml / 394.8 = 8.87 x 10^-3 ml = 8.87 ul
3.5 ml / 0.150 = 23.3 fold; 1.692 x 23.3 = 39.4 ul
1.692/0.150 = 11.28 fold; 3.5 ml / 11.28 = 0.310 ml = 310 ul](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1addf139-0498-4e11-bca0-a0c7874b4001%2F70048fc4-89ed-4dcd-877f-cd3875d31092%2Fw5th43n_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Question 6
A stock solution of Methylene Blue dye has absorbance (A) of 1.692 at 668 nm. How many fold (times) do you
need to dilute this solution to obtain a working solution having absorbance of 0.150? How many microliters (ul)
of the stock solution will be necessary to prepare 3.5 ml of the working solution?
3
668/ 1.692 394.8 fold; 3.5 ml / 394.8 = 8.87 x 10^-3 ml = 8.87 ul
3.5 ml / 0.150 = 23.3 fold; 1.692 x 23.3 = 39.4 ul
1.692/0.150 = 11.28 fold; 3.5 ml / 11.28 = 0.310 ml = 310 ul
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