A stock solution of Methylene Blue dye has absorbance (A) of 1.692 at 668 nm. How many fold (times) do you need to dilute this solution to obtain a working solution having absorbance of 0.150? How many microliters (ul) of the stock solution will be necessary to prepare 3.5 ml of the working solution? 668/1.692 394.8 fold; 3.5 ml / 394.8 8.87 x 10^-3 ml = 8.87 ul 3.5 ml / 0.150 = 23.3 fold; 1.692 x 23.3 = 39.4 ul 1.692/0.150 = 11.28 fold; 3.5 ml / 11.28 = 0.310 ml = 310 ul

A stock solution of Methylene Blue dye has absorbance (A) of 1.692 at 668 nm. How many fold (times) do you need to dilute this solution to obtain a working solution having absorbance of 0.150? How many microliters (ul) of the stock solution will be necessary to prepare 3.5 ml of the working solution? 668/1.692 394.8 fold; 3.5 ml / 394.8 8.87 x 10^-3 ml = 8.87 ul 3.5 ml / 0.150 = 23.3 fold; 1.692 x 23.3 = 39.4 ul 1.692/0.150 = 11.28 fold; 3.5 ml / 11.28 = 0.310 ml = 310 ul

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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