QUESTION 1 You obtained the following raw data when setting up a Bluret standard curve BSA (mgim) Absorbancy 540nm 0158 0210 0260 3 0305 4. 0360 0.410 0455 7. 0510 0530 0550 10 0554 Ater blanking against a biuret d0 sample. the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0324 was obtained Set up a standard curve excluding outiers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures)
QUESTION 1 You obtained the following raw data when setting up a Bluret standard curve BSA (mgim) Absorbancy 540nm 0158 0210 0260 3 0305 4. 0360 0.410 0455 7. 0510 0530 0550 10 0554 Ater blanking against a biuret d0 sample. the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0324 was obtained Set up a standard curve excluding outiers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures)
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![QUESTION 1
You obtained the following raw data when setting up a Bkuret standard curve
BSA (mg/ml)
Absorbancy
540nm
0.158
0210
0.260
3
0 305
4.
0360
0.410
6
0 455
0.510
0 530
0.550
10
0 554
After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.324 was obtained
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / mil (up to 3 significant figures).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fbad36464-4e5e-43b7-921a-6395099007d8%2Fafb28c6c-1b44-427d-a915-c690328c9a3b%2Fy7w31m5_processed.jpeg&w=3840&q=75)
Transcribed Image Text:QUESTION 1
You obtained the following raw data when setting up a Bkuret standard curve
BSA (mg/ml)
Absorbancy
540nm
0.158
0210
0.260
3
0 305
4.
0360
0.410
6
0 455
0.510
0 530
0.550
10
0 554
After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.324 was obtained
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / mil (up to 3 significant figures).
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