QUESTION 1 You obtained the following raw data when setting up a Bluret standard curve BSA (mgim) Absorbancy 540nm 0158 0210 0260 3 0305 4. 0360 0.410 0455 7. 0510 0530 0550 10 0554 Ater blanking against a biuret d0 sample. the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0324 was obtained Set up a standard curve excluding outiers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures)

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QUESTION 1
You obtained the following raw data when setting up a Bkuret standard curve
BSA (mg/ml)
Absorbancy
540nm
0.158
0210
0.260
3
0 305
4.
0360
0.410
6
0 455
0.510
0 530
0.550
10
0 554
After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.324 was obtained
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / mil (up to 3 significant figures).
Transcribed Image Text:QUESTION 1 You obtained the following raw data when setting up a Bkuret standard curve BSA (mg/ml) Absorbancy 540nm 0.158 0210 0.260 3 0 305 4. 0360 0.410 6 0 455 0.510 0 530 0.550 10 0 554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.324 was obtained Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / mil (up to 3 significant figures).
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