Question 1: Using a well-known protein estimation technique, absorbance reading for a set of standards were obtained. You were required to plot the data. Which graph would you use? Plot the data using the graph type. Protein concentration Absorbance (a.u.) (mg/mL) 0.01 2 0.05 0.12 10 0.25 25 0.63 50 1.25 Question 2: Enzymes are biological catalysts and perform important biochemical reactions in the body. A group of scientists discovered a new enzyme – Enzyme X. To understand the working of this enzyme, they performed biochemical assays to assess the activity of the enzyme. The results for which are shown in the table below. Based on the information provided in the table, please state which graph would you use? Plot the data using the graph type. Enzyme activity (%) 35 pH 3. 55 68 79 98 8. 85 9. 20 10 4667 coa

Question 1: Using a well-known protein estimation technique, absorbance reading for a set of standards were obtained. You were required to plot the data. Which graph would you use? Plot the data using the graph type. Protein concentration Absorbance (a.u.) (mg/mL) 0.01 2 0.05 0.12 10 0.25 25 0.63 50 1.25 Question 2: Enzymes are biological catalysts and perform important biochemical reactions in the body. A group of scientists discovered a new enzyme – Enzyme X. To understand the working of this enzyme, they performed biochemical assays to assess the activity of the enzyme. The results for which are shown in the table below. Based on the information provided in the table, please state which graph would you use? Plot the data using the graph type. Enzyme activity (%) 35 pH 3. 55 68 79 98 8. 85 9. 20 10 4667 coa

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus concentration (in M) of known solutions and finds the following slope and intercept: y = 1.646x + 0.026 Sally finds that her original Kool-aid sample is too concentrated, so they dilute it by transferring 13.00 mL of the original Kool-aid sample into a new container and diluting to a total volume of 37.00 mL. If the absorbance of the dilute Kool-aid sample at the appropriate wavelength for their assigned dye is 0.950, determine the concentration (in M) of the assigned food dye in the original Kool-aid sample. Report answer with three places after the decimal.
You have prepared several dilutions of a standard compound. You measure the absorbance of each solution at 340 nm using a 1 cm cuvette and your results are listed in the table below. Determine the concentration of an unknown sample with an absorbance of 0.085 at 340 nm. Concentration (uM) Absorbance at 340 nm 2. 0.009 4. 0.020 0.061 16 0.111 32 0.189
Let's pretend your little cousin walked in on you as you were performing your experiment. They took your dyed liquid and made their own sample by diluting it with water just as you did. However you did not see how much water they added. Assume the graph below represents your measured results. Now lets say you take one more image using your little cousin's sample of unknown concentration, and you calculate the absorption value to be 0.34. What is the approximate concentration of the solution that your little cousin mixed?
You want to figure out the caffeine concentration in a coffee you bought. You make four caffeine standard solutions of increasing concentration and fit the data. Your line fit has the following equation: y = 0.053(AU/mM) X + 0.0012AU You test your coffee sample and measure an absorbance of 0.82 AU. What is the concentration ○ 0.0042 mM ○ 0.0042 M ○ 15 mM ○ 0.043 mM ○ 15 M 0.043 M
Determination of an Equilibrium Constant Data Beer's Law constant Determination Beakers (Fe(SCN)-] Absorbance Measured OM 0.0002 M 0.00016 M 0.00012 M 0.00008 M Known 0.676 0.556 4 0.390 5 0.196 6. 0.298 Beer's Law Plot: Turn in the graph you produced in Excel The value of absorptivity constant (k) is. What is the concentration of your unknown, knowing the measured absorbance was found to be 0.298?
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Absorbance Beer-Lambert's Law (Spectrophotometry) #2 To spectrophotometrically determine the mass percent of cobalt in an ore containing cobalt and some inert materials, solutions with known [Co"] are prepared and the absorbance of each of the solutions is measured at the wavelength of optimum absorbance. The data are used to create a calibration plot, shown below. 0.80 0.70- 8 0.60- 0.50- 0.40- 0.30 0.20- 0.10- ++- 0.015 Concentration (M) A 0.6 g sample of the ore is completely dissolved in concentrated HNO3(aq). The mixture is diluted with 2+ water to a final volume of 50.00 mL. Assume that all the cobalt in the ore sample is converted to Co'(aq). a. What is the (Co] in the solution if the absorbance of a sample of the solution is 0.74? b. Calculate the number of moles of CoT(ag) in the 50.00 mL solution. 2+ C. Calculate the mass percent of Co in the 0.630 g sample of the ore.
Given this data, if a CV solution has an absorbance of 1.125, what is [CV]? [CV] / M A 1.0 × 10–6 M 0.250 3.0 × 10–6 M 0.750 Question 12 options: 4.5 × 10–6 M 5.0 × 10–6 M 5.5 × 10–6 M
em1.png Part 3: Q9 Average absorbance Flask 8 Flask 9 Flask 10 1:1 Ratio so, Flask 11 Part 3: Q Flask 8 Flask 9 Flask 10 Flask 11 Part 3: Q10 Flask 8 Flask 9 Flask 10 Flask 11 Part 3 0.146 0.166 [Fe] -0.002mol/L 0.215 0.294 [FESCN³mol/L) 4.59% 10 5.18*10* 6.64*10* 9.08*10* [FXmol/L) 5.00 *10* 5.00*10* Y=3362x-0.0083 R² = 0.9993 5.00*10* 5.00*10* (Amounts of flask 8 (9 - 11's values are in the spreadsheet) 71% volume 5.00mL Molarity of Fe(NO3))3 = 0.2M Volume of Fe(NO3))3 = 5.00mL Moles of Fe³= Molarity x volume(L) Moles of Fe¹ = 0.2M X 0.005L = 1x10-³mole x [SCN][mol/ 4x10 5×10-4 7x10 1×10 1 Concentration of Fe³ = mole of Fe³ Volume mole of Fe³1x10³ mole G.O05L 8, Average absorbance for solution 8 (same for 9 - 11) Average absorbance for flask 8 = sum of all absorbances / number of readings (0.145 +0.147 +0.148) 3 Using equation of calibration curve's line(slope) of best fit, the average absorbances of the unknown concentrations to determine [FeSCN-Jat equilibrium in solution 8:…
Chemistry
The concentration of methylene blue in the sample is estimated through (select the correct) options: refractive index measurement (refractometry). turbidity measurement (turbidimetry). absorbance measurement (spectrophotometry). titration with 2% HCl solution.
A medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985. y = 0.0523 x + 0.072 A patient sample has an absorbance of 0.162. Using the standard curve data, calculate the percent hemoglobin in the patient sample