كرة HPLCQ3: The Figure shown a HPLC chromatogram of two compound .Calculate thefollowing values using the data in this chromatogram.Detector signala. The adjusted retention time of uracil and guanine.b. The peak widths for uracil and guanine.The retention factor for uracil and guanine.C.d.The selectivity factor for uracil and guanine.e. The resolution for these two compounds.f. The column efficiency parameters, N and H, if the column length is 10cm.0.6-0504030.20.1-45.0 svoldA15 30 45تختبر تحليل الى180.0 sAuracil9060 762475sguanine105-120 135 150 166 180 195 210 225 240 255 270 296Time, seconds

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Q3: The Figure shown a HPLC chromatogram of two compound.Calculate the following values using the data in this chromatogram. Detector signal a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. c. The retention factor for uracil and guanine. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 0.6- 05- 04 03- 02- 0.1 15 30 45.0 s void 45 60 76 كليل الك 90 1800 s uracil 247.5s guanine 105 120 135 150 166 180 195 210 225 240 255 270 296 Time, seconds

Q3: The Figure shown a HPLC chromatogram of two compound.Calculate the following values using the data in this chromatogram. Detector signal a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. c. The retention factor for uracil and guanine. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 0.6- 05- 04 03- 02- 0.1 15 30 45.0 s void 45 60 76 كليل الك 90 1800 s uracil 247.5s guanine 105 120 135 150 166 180 195 210 225 240 255 270 296 Time, seconds

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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