Why is the spectrophotometer set at 100% T using the FeCl3 solution rather than distilled water? The iron chloride solution is used as the blank bećause aspirin is not soluble in distilled water. The iron chloride solution is used as the blank because distilled water will interfere with the absorbance readings. The iron chloride solution is used as the blank because that is the solvent which is present for all solutions. O The iron chloride solution is used as the blank because distilled water has no color.

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**Educational Content: Spectrophotometry and Solution Preparation**

**Why is the spectrophotometer set at 100% T using the FeCl₃ solution rather than distilled water?**

- ◯ The iron chloride solution is used as the blank because aspirin is not soluble in distilled water.
- ◯ The iron chloride solution is used as the blank because distilled water will interfere with the absorbance readings.
- ◯ The iron chloride solution is used as the blank because that is the solvent which is present for all solutions.
- ◯ The iron chloride solution is used as the blank because distilled water has no color.

**Explanation:**
In spectrophotometry, selecting an appropriate blank (or reference) solution is crucial for accurate measurement of absorbance. The blank should match the solvent used in the sample solutions to ensure that any absorbance measured is due to the analyte, and not the solvent or other components.

**Reasoning Through Each Option:**

- The first option suggests that aspirin is not soluble in distilled water, indicating a reason for not using distilled water as a solvent or blank.
- The second option implies that distilled water might affect the absorbance readings, possibly due to an interaction with the analyte or its own absorbance characteristics.
- The third option states that iron chloride solution is universally the solvent in all sample solutions, justifying its use as the blank to negate its absorbance and focus on the analyte's absorbance only.
- The fourth option discounts distilled water due to its lack of color, though this is less likely a primary reason compared to the solvent consistency provided by iron chloride solution.

Choosing the correct blank is essential to achieve accurate and precise spectrophotometric data, ensuring that the measurements reflect the analyte's properties rather than any extraneous variables introduced by differing solvents.
Transcribed Image Text:**Educational Content: Spectrophotometry and Solution Preparation** **Why is the spectrophotometer set at 100% T using the FeCl₃ solution rather than distilled water?** - ◯ The iron chloride solution is used as the blank because aspirin is not soluble in distilled water. - ◯ The iron chloride solution is used as the blank because distilled water will interfere with the absorbance readings. - ◯ The iron chloride solution is used as the blank because that is the solvent which is present for all solutions. - ◯ The iron chloride solution is used as the blank because distilled water has no color. **Explanation:** In spectrophotometry, selecting an appropriate blank (or reference) solution is crucial for accurate measurement of absorbance. The blank should match the solvent used in the sample solutions to ensure that any absorbance measured is due to the analyte, and not the solvent or other components. **Reasoning Through Each Option:** - The first option suggests that aspirin is not soluble in distilled water, indicating a reason for not using distilled water as a solvent or blank. - The second option implies that distilled water might affect the absorbance readings, possibly due to an interaction with the analyte or its own absorbance characteristics. - The third option states that iron chloride solution is universally the solvent in all sample solutions, justifying its use as the blank to negate its absorbance and focus on the analyte's absorbance only. - The fourth option discounts distilled water due to its lack of color, though this is less likely a primary reason compared to the solvent consistency provided by iron chloride solution. Choosing the correct blank is essential to achieve accurate and precise spectrophotometric data, ensuring that the measurements reflect the analyte's properties rather than any extraneous variables introduced by differing solvents.
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