Procedure 1. Place 2mL of milk in each of the 3 test tubes. 2. Heat one of the test tubes to boiling and cool. 3. Add 5 drops of 1% Methylene blue solution to each test tube. 4. Add 2 drops of 1% Formaldehyde to the test tube containing boiled milk and to the other containing unboiled milk leaving the third tube as a control. 5. Mix well. 6. Add 1mL of Paraffin oil to each test tube and warm in a water bath for 10-15 minutes at 40°C. 7. Observe the change that is taking place in each test tube.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Experiment: Animal Oxidase

Procedures are indicated below. (Please view the image)

Question: What will be the possible result and observation? What's the principle behind the results?

Animal Oxidase
Procedure
1. Place 2mL of milk in each of the 3 test tubes.
2. Heat one of the test tubes to boiling and cool.
3. Add 5 drops of 1% Methylene blue solution to each test tube.
4. Add 2 drops of 1% Formaldehyde to the test tube containing boiled milk and to the other
containing unboiled milk leaving the third tube as a control.
5. Mix well.
6. Add 1mL of Paraffin oil to each test tube and warm in a water bath for 10-15 minutes at 40°C.
7. Observe the change that is taking place in each test tube.
Transcribed Image Text:Animal Oxidase Procedure 1. Place 2mL of milk in each of the 3 test tubes. 2. Heat one of the test tubes to boiling and cool. 3. Add 5 drops of 1% Methylene blue solution to each test tube. 4. Add 2 drops of 1% Formaldehyde to the test tube containing boiled milk and to the other containing unboiled milk leaving the third tube as a control. 5. Mix well. 6. Add 1mL of Paraffin oil to each test tube and warm in a water bath for 10-15 minutes at 40°C. 7. Observe the change that is taking place in each test tube.
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