Problem Set on Enzyme Kinetics Phosphofructokinase (PFK) is a major control point in regulating the flux of metabolites into the glycolysis. A Biochemist has successfully isolated a potent specific inhibitor of PFK (inhibitor A) from an endemic plant in the Philippines. The following data describe the effect of the purified inhibitor on the activity of PFK. (20 points) Without Inhibitor With Inhibitor A [S] (mM) (μM⚫s¯¹) (μM⚫s¯¹) 1 2.5 1.17 10 2502 4.0 2.10 6.3 4.00 7.6 5.7 9.0 7.2 A. Construct a Michaelis-Menten and Lineweaver-Burke curves for the kinetic data above in the absence and presence of inhibitor A. B. Determine the Km, Km.app. Vmax, Xmax.app from the kinetic data. Show your complete calculation and encircle your final answers. Determine the mode of inhibition exhibited by inhibitor A Discuss your answer briefly. C. If the total PFK concentration is 1 nmol/L, how many molecules of substrate can a molecule of PFK process in each minute in the absence and presence of inhibitor A. Show your complete calculation and encircle your final answers. D. Determine the catalytic efficiency of PFK (in the absence and presence of inhibitor A. Is PFK a fairly efficient enzyme? Show your complete calculation and encircle your final answers.
Problem Set on Enzyme Kinetics Phosphofructokinase (PFK) is a major control point in regulating the flux of metabolites into the glycolysis. A Biochemist has successfully isolated a potent specific inhibitor of PFK (inhibitor A) from an endemic plant in the Philippines. The following data describe the effect of the purified inhibitor on the activity of PFK. (20 points) Without Inhibitor With Inhibitor A [S] (mM) (μM⚫s¯¹) (μM⚫s¯¹) 1 2.5 1.17 10 2502 4.0 2.10 6.3 4.00 7.6 5.7 9.0 7.2 A. Construct a Michaelis-Menten and Lineweaver-Burke curves for the kinetic data above in the absence and presence of inhibitor A. B. Determine the Km, Km.app. Vmax, Xmax.app from the kinetic data. Show your complete calculation and encircle your final answers. Determine the mode of inhibition exhibited by inhibitor A Discuss your answer briefly. C. If the total PFK concentration is 1 nmol/L, how many molecules of substrate can a molecule of PFK process in each minute in the absence and presence of inhibitor A. Show your complete calculation and encircle your final answers. D. Determine the catalytic efficiency of PFK (in the absence and presence of inhibitor A. Is PFK a fairly efficient enzyme? Show your complete calculation and encircle your final answers.
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter24: Lipid Biosynthesis
Section: Chapter Questions
Problem 4P
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![Problem Set on Enzyme Kinetics
Phosphofructokinase (PFK) is a major control point in regulating the flux of metabolites
into the glycolysis. A Biochemist has successfully isolated a potent specific inhibitor of
PFK (inhibitor A) from an endemic plant in the Philippines. The following data describe
the effect of the purified inhibitor on the activity of PFK. (20 points)
Without Inhibitor With Inhibitor A
[S]
(mM)
(μM⚫s¯¹)
(μM⚫s¯¹)
1
2.5
1.17
10
2502
4.0
2.10
6.3
4.00
7.6
5.7
9.0
7.2
A.
Construct a Michaelis-Menten and Lineweaver-Burke curves for the kinetic data
above in the absence and presence of inhibitor A.
B. Determine the Km, Km.app. Vmax, Xmax.app from the kinetic data. Show your
complete calculation and encircle your final answers. Determine the mode of inhibition
exhibited by inhibitor A Discuss your answer briefly.
C. If the total PFK concentration is 1 nmol/L, how many molecules of substrate can
a molecule of PFK process in each minute in the absence and presence of inhibitor A.
Show your complete calculation and encircle your final answers.
D. Determine the catalytic efficiency of PFK (in the absence and presence of
inhibitor A. Is PFK a fairly efficient enzyme? Show your complete calculation and
encircle your final answers.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4328c9cc-6fa0-407f-9af0-e59e5fb1715c%2Ff599daf8-6bf9-493a-afc3-44eaee0728aa%2F80wx0rn_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Problem Set on Enzyme Kinetics
Phosphofructokinase (PFK) is a major control point in regulating the flux of metabolites
into the glycolysis. A Biochemist has successfully isolated a potent specific inhibitor of
PFK (inhibitor A) from an endemic plant in the Philippines. The following data describe
the effect of the purified inhibitor on the activity of PFK. (20 points)
Without Inhibitor With Inhibitor A
[S]
(mM)
(μM⚫s¯¹)
(μM⚫s¯¹)
1
2.5
1.17
10
2502
4.0
2.10
6.3
4.00
7.6
5.7
9.0
7.2
A.
Construct a Michaelis-Menten and Lineweaver-Burke curves for the kinetic data
above in the absence and presence of inhibitor A.
B. Determine the Km, Km.app. Vmax, Xmax.app from the kinetic data. Show your
complete calculation and encircle your final answers. Determine the mode of inhibition
exhibited by inhibitor A Discuss your answer briefly.
C. If the total PFK concentration is 1 nmol/L, how many molecules of substrate can
a molecule of PFK process in each minute in the absence and presence of inhibitor A.
Show your complete calculation and encircle your final answers.
D. Determine the catalytic efficiency of PFK (in the absence and presence of
inhibitor A. Is PFK a fairly efficient enzyme? Show your complete calculation and
encircle your final answers.
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