PO mod 5' 787 18 32 806 789 bases P3 mod P5 3' 1542 1507 1487 721 bases FIG. 1. Diagram of locations of primers on Escherichia coli 16S rDNA, an open reading frame that is 1,542 bases long. The locations of the modified priming sites used for the PCR are shown. Pomod primes at site PO with an extension going to the right. P3mod primes at site P3 with the reaction going to the right; PC3mod primes at the same site with the reaction going to the left. PC5 primes at site P5 with the reaction going to the left. The designations 5' and 3' and base numbering refer to the sense strand.
PO mod 5' 787 18 32 806 789 bases P3 mod P5 3' 1542 1507 1487 721 bases FIG. 1. Diagram of locations of primers on Escherichia coli 16S rDNA, an open reading frame that is 1,542 bases long. The locations of the modified priming sites used for the PCR are shown. Pomod primes at site PO with an extension going to the right. P3mod primes at site P3 with the reaction going to the right; PC3mod primes at the same site with the reaction going to the left. PC5 primes at site P5 with the reaction going to the left. The designations 5' and 3' and base numbering refer to the sense strand.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
-
Figure 1 from Wilson et al. (1990) shows the locations of the primers and the length of the PCR fragments for Escherichia coli. Redraw Figure 1 to show the primer locations and the PCR fragments lengths as it would be for Coxiella burnettii. This image is from the
PCR Article - Wilson et al (1990) article
Transcribed Image Text:PO mod
5'
787
18
32
806
789 bases
P3 mod
P5
3'
1542
1507
1487
721 bases
FIG. 1. Diagram of locations of primers on Escherichia coli 16S rDNA, an open reading frame that is 1,542 bases long. The locations of
the modified priming sites used for the PCR are shown. Pomod primes at site PO with an extension going to the right. P3mod primes at site
P3 with the reaction going to the right; PC3mod primes at the same site with the reaction going to the left. PC5 primes at site P5 with the
reaction going to the left. The designations 5' and 3' and base numbering refer to the sense strand.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education