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Penicillin G reacts with hydroxylamine (NH2OH) to form hydroxamic acid which produces a coloured complex with iron (III). To determine the overall reaction order, equal concentrations of penicillin and NH2OH were mixed in a 250 ml flask. Samples were withdrawn every 10 minutes and added to a solution containing iron (III) chloride. With the aid of a colorimeter, the concentration of the coloured complex and hence the concentration of hydroxamic acid was obtained as a function of time. The absorbance shown in the table below is directly proportional to the hydroxamic acid concentration.

Penicillin G reacts with hydroxylamine (NH2OH) to form hydroxamic acid which produces a coloured complex with iron (III). To determine the overall reaction order, equal concentrations of penicillin and NH2OH were mixed in a 250 ml flask. Samples were withdrawn every 10 minutes and added to a solution containing iron (III) chloride. With the aid of a colorimeter, the concentration of the coloured complex and hence the concentration of hydroxamic acid was obtained as a function of time. The absorbance shown in the table below is directly proportional to the hydroxamic acid concentration.

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
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Penicillin G reacts with hydroxylamine (NH2OH) to form hydroxamic acid which produces a coloured complex with iron (III). To determine the overall reaction order, equal concentrations of penicillin and NH2OH were mixed in a 250 ml flask. Samples were withdrawn every 10 minutes and added to a solution containing iron (III) chloride. With the aid of a colorimeter, the concentration of the coloured complex and hence the concentration of hydroxamic acid was obtained as a function of time. The absorbance shown in the table below is directly proportional to the hydroxamic acid concentration. Determine the overall reaction order and calculate the reaction specific rate constant assuming an irreversible reaction. Time (min) 10 20 30 40 50 Absorbance 0.0 0.337 0.433 0.495 0.539 0.561 0.685
Transcribed Image Text:Penicillin G reacts with hydroxylamine (NH2OH) to form hydroxamic acid which produces a coloured complex with iron (III). To determine the overall reaction order, equal concentrations of penicillin and NH2OH were mixed in a 250 ml flask. Samples were withdrawn every 10 minutes and added to a solution containing iron (III) chloride. With the aid of a colorimeter, the concentration of the coloured complex and hence the concentration of hydroxamic acid was obtained as a function of time. The absorbance shown in the table below is directly proportional to the hydroxamic acid concentration. Determine the overall reaction order and calculate the reaction specific rate constant assuming an irreversible reaction. Time (min) 10 20 30 40 50 Absorbance 0.0 0.337 0.433 0.495 0.539 0.561 0.685
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