P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces maximally at 510 nm, can be used to label amino acids in fluorescence microscopy and FRET studies. Tabulated below is the variation of the fluorescence intensity of an aqueous solution of dansyl chloride with time after excitation by a short laser pulse (with I, the initial fluorescence intensity The ratio of intensities is equal to the ratio of the rates of photon emission. t/ns 5.0 10.0 15.0 20.0 0.45 0.21 0.11 0.05

P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces maximally at 510 nm, can be used to label amino acids in fluorescence microscopy and FRET studies. Tabulated below is the variation of the fluorescence intensity of an aqueous solution of dansyl chloride with time after excitation by a short laser pulse (with I, the initial fluorescence intensity The ratio of intensities is equal to the ratio of the rates of photon emission. t/ns 5.0 10.0 15.0 20.0 0.45 0.21 0.11 0.05

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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