P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces maximally at 510 nm, can be used to label amino acids in fluorescence microscopy and FRET studies. Tabulated below is the variation of the fluorescence intensity of an aqueous solution of dansyl chloride with time after excitation by a short laser pulse (with I, the initial fluorescence intensity The ratio of intensities is equal to the ratio of the rates of photon emission. t/ns 5.0 10.0 15.0 20.0 0.45 0.21 0.11 0.05
P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces maximally at 510 nm, can be used to label amino acids in fluorescence microscopy and FRET studies. Tabulated below is the variation of the fluorescence intensity of an aqueous solution of dansyl chloride with time after excitation by a short laser pulse (with I, the initial fluorescence intensity The ratio of intensities is equal to the ratio of the rates of photon emission. t/ns 5.0 10.0 15.0 20.0 0.45 0.21 0.11 0.05
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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![P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces
maximally at 510 nm, can be used to label amino acids in fluorescence
microscopy and FRET studies. Tabulated below is the variation of the
fluorescence intensity of an aqueous solution of dansyl chloride with time
after excitation by a short laser pulse (with I, the initial fluorescence intensity).
The ratio of intensities is equal to the ratio of the rates of photon emission.
t/ns
5.0
10.0
15.0
20.0
II.
0.45
0.21
0.11
0.05
(a) Calculate the observed fluorescence lifetime of dansyl chloride in water.
(b) The fluorescence quantum yield of dansyl chloride in water is 0.70. What
is the fluorescence rate constant?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ffe0776c3-4461-42af-a9c6-2a84d358635d%2F7acf7a13-33d6-4cc1-a4aa-40a8807f4bb4%2Fh0hxhvp_processed.jpeg&w=3840&q=75)
Transcribed Image Text:P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces
maximally at 510 nm, can be used to label amino acids in fluorescence
microscopy and FRET studies. Tabulated below is the variation of the
fluorescence intensity of an aqueous solution of dansyl chloride with time
after excitation by a short laser pulse (with I, the initial fluorescence intensity).
The ratio of intensities is equal to the ratio of the rates of photon emission.
t/ns
5.0
10.0
15.0
20.0
II.
0.45
0.21
0.11
0.05
(a) Calculate the observed fluorescence lifetime of dansyl chloride in water.
(b) The fluorescence quantum yield of dansyl chloride in water is 0.70. What
is the fluorescence rate constant?
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