Mulecular Absorption SpectrometryMacBook Pro6. Describe the advantage of a four-level laser system over a three-level type.7.The reduced form of nicotinamide adenine dinucleotide (NADH) is an importantand highly fluorescent coenzyme. It has an absorption maximum of 340 nm and anemission maximum at 465 nm. Standard solutions of NADH gave the followingfluorescence intensities:Relative IntensityConc. NADH, umol/L0.1002.240.2004.520.3006.639.010.4000.50010.940.60013.710.70015.490.80017.91A) Construct a spreadsheet and use it to draw a calibration curve for NADH.B) Find the least-squares slope and intercept for the plot in (A)C) An unknown exhibit a relative fluorescence intensity of 12.16. Use the spreadsheetto calculate the concentration of NADH.8. The IR spectrum of CO shows a vibrational absorption band centered at 2170/cm.(a) what is the force constant for the CO bond?(b) At what wavenumber would the corresponding peak for "CO occur?9. Calculate the relative number of protons in the higher and lower magnetic states wheresample is placed in a 5T field at 25°C.10. Calculate the energy (in kJ/mol) that electrons acquire as a result of being acceleratedthrough a potential of 80 V. (b) How does this energy compare to that of a typicalchemical bond?

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7. The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities:

7. The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities:

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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