Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.
Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter18: Raman Spectroscopy
Section: Chapter Questions
Problem 18.7QAP
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