Homework Help is Here – Start Your Trial Now!
Science
Biology
ntrolling the cell cycle. Earlier researchers ha frog eggs (Xenopus) contained all the necess ired for DNA replication. This included protei noting factor (MPF). At the time of this study, ity and the research group wanted to test usi 1. In Figure 1 (a) MPF activity was tested for it ne (H1 in snerm chro matin ove hromatin ortain p

ntrolling the cell cycle. Earlier researchers ha frog eggs (Xenopus) contained all the necess ired for DNA replication. This included protei noting factor (MPF). At the time of this study, ity and the research group wanted to test usi 1. In Figure 1 (a) MPF activity was tested for it ne (H1 in snerm chro matin ove hromatin ortain p

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
In the following study, the investigators wanted to determine the role of cyclin B in controlling the cell cycle. Earlier researchers had found that extracts made from frog eggs (Xenopus) contained all the necessary proteins and machinery required for DNA replication. This included proteins that regulated the mitosis promoting factor (MPF). At the time of this study, cyclin B was show to affect MPF activity and the research group wanted to test using Xenopus egg extract in an assay. In Figure 1 (a) MPF activity was tested for its ability to phosphorylate Histone (H1) in sperm chromatin over a certain period of time. Additionally, the cyclin B concentration in the extract was measured. In figure 1b, the extract was tested after treatment with RNase which degraded only the mRNA and not RNA or FRNA in the extract. Knowing that cyclin B is a short-lived protein, why do you suppose the graph shows the results you see in figure 1b?
Transcribed Image Text:In the following study, the investigators wanted to determine the role of cyclin B in controlling the cell cycle. Earlier researchers had found that extracts made from frog eggs (Xenopus) contained all the necessary proteins and machinery required for DNA replication. This included proteins that regulated the mitosis promoting factor (MPF). At the time of this study, cyclin B was show to affect MPF activity and the research group wanted to test using Xenopus egg extract in an assay. In Figure 1 (a) MPF activity was tested for its ability to phosphorylate Histone (H1) in sperm chromatin over a certain period of time. Additionally, the cyclin B concentration in the extract was measured. In figure 1b, the extract was tested after treatment with RNase which degraded only the mRNA and not RNA or FRNA in the extract. Knowing that cyclin B is a short-lived protein, why do you suppose the graph shows the results you see in figure 1b?
Untreated extract (b) RNase-treated axtract Mitotic events -- MPF activity - Cyclin B cone Sperm chromatin Time Time > RNase-treated extract + wild-type cyclin B MRNA (d) RNase-treated extract + nondegradable cyclin B MRNA Mitotic events Sperm chromatin Time Mitotic arrest Time > IGURE 13-7 Experimental demonstration that the thesis and degradation of cyclin B are required for the ing of MPF activity and mitotic events in Xenopus egg acts. In all cases, MPF activity and cyclin B concentration a determined at various times after addition of sperm matin to an extract treated as indicatod. Microscopic vations detorminod the occurrence of early mitotic events (blue shading), including chromosome condensation and nuclear envelope breakdown, and of late events (orange shading). including chromosome decondensation and nuclear envelope reformation. Soe text for discussion. ISee A. W. Murray et al, 1989, Nature 339:275; adapted from A. Murray and T, Hunt, 1993, The Cel Cycle: An introduction, W. H. Freeman and Company)
Transcribed Image Text:Untreated extract (b) RNase-treated axtract Mitotic events -- MPF activity - Cyclin B cone Sperm chromatin Time Time > RNase-treated extract + wild-type cyclin B MRNA (d) RNase-treated extract + nondegradable cyclin B MRNA Mitotic events Sperm chromatin Time Mitotic arrest Time > IGURE 13-7 Experimental demonstration that the thesis and degradation of cyclin B are required for the ing of MPF activity and mitotic events in Xenopus egg acts. In all cases, MPF activity and cyclin B concentration a determined at various times after addition of sperm matin to an extract treated as indicatod. Microscopic vations detorminod the occurrence of early mitotic events (blue shading), including chromosome condensation and nuclear envelope breakdown, and of late events (orange shading). including chromosome decondensation and nuclear envelope reformation. Soe text for discussion. ISee A. W. Murray et al, 1989, Nature 339:275; adapted from A. Murray and T, Hunt, 1993, The Cel Cycle: An introduction, W. H. Freeman and Company)
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Cell division
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS