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Name the technique which is used to detect HIV in Suspected AIDS patient?
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- Name the technique based on the principle of antigen-antibody interaction used in detection of a virus (HIV).All of the following are true concerning the Alere HIV test except: a)this test can detect infection 10 days post exposure b)the presence of three lines on the test indicates a positive reaction for anitgen and antibody against HIV c)unlike Oraquick the test is run with blood sample only and not oral fluid d)the presence of a single line indicates that a person has antibodies but no antigen for HIV e)this is a test that is considered both a direct and indirect test for HIV diagnosisPrior to the development of detection assay for HIV in the blood supply, HCV was used as a proxy. That is to say, any donated blood found to be contaminated with HBV or HCV was also assumed to be HIV positive as well. What do you think was the rationale behind the use of HCV as an indicator of HIV? Do you think this was a reasonable approach at the time?
- Why is a false-positive more significant in HIV testing of patients than in screening donated blood for transfusions?Describe an ELISA test to detect the presence of HIV antibodies in a patient.List two of the major targets of combination antiretroviral therapies used to clinically treat HIV-1 infections.
- To diagnose someone with HIV, what do they look for in the blood?What are the main biomarkers in the blood that can be used to diagnose HIV infection? Give at least one advantage and one disadvantage for each biomarker. What tests are available to detect each of these biomarkers?Describe the applications of BCG vaccine.