NaCl (Mr = 58.443 g/mol) 2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10 % Sodium dodecyl sulfate solution Proteinase K solution (50 mg dissolved in 1 ml ddH2O) You need a digestion buffer consisting of the following: 15 mM NaCl 75 mM Tris-Cl, pH 8 16 mM EDTA, pH 8 0.8 % Sodium dodecyl sulfate 0.75 mg/ml proteinase K Calculate: How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations and remember to explain how exactly you will make it up.
NaCl (Mr = 58.443 g/mol) 2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10 % Sodium dodecyl sulfate solution Proteinase K solution (50 mg dissolved in 1 ml ddH2O) You need a digestion buffer consisting of the following: 15 mM NaCl 75 mM Tris-Cl, pH 8 16 mM EDTA, pH 8 0.8 % Sodium dodecyl sulfate 0.75 mg/ml proteinase K Calculate: How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations and remember to explain how exactly you will make it up.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 8SST
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NaCl (Mr = 58.443 g/mol)
2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre)
EDTA, sodium salt (Mr = 380.2 g/mol)
10 % Sodium dodecyl sulfate solution
Proteinase K solution (50 mg dissolved in 1 ml ddH2O)
You need a digestion buffer consisting of the following:
15 mM NaCl
75 mM Tris-Cl, pH 8
16 mM EDTA, pH 8
0.8 % Sodium dodecyl sulfate
0.75 mg/ml proteinase K
Calculate:
How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations and remember to explain how exactly you will make it up.
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