Answered: Minimize pipette handling Questions :…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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ANSWER THE QUESTION PROPERLY INTRODUCTION : Pipetting is one of the most important skill of a cell and molecular biologist. This technique is needed because you will be working in the molecular scale. You will be measuring minute amount of liquid and other toxic / and non-toxic materials. You will also be working with DNA samples and other materials that must be transferred from one micro-container into another. This activity will help you develop your skills in pipetting as a major skill to be acquired in this subject matter. As your laboratory’s demand for accuracy and precision grows, so does the importance of understanding and developing an optimal pipetting technique. Walk through the following 12 steps that will bring your pipetting to the next level, and see how many you have already mastered and integrated as part of your everyday pipetting routine. Step 1: Pre-wet the pipette Step 2: Immerse the tip to the proper depth during aspiration Step 3: Pause consistently after aspiration Step 4: Use consister plunger pressure and speed Step 5: Pull the Pipette straight out Step 6: Examine the tip BEFORE dispensing a sample Step 7: Examine thr tip AFTER dispening a sample Step 8: Use standard mode pipetting Step 9: Use the appropriate pipette Step 10: Use thr correct pipette tip Step 11: Work at ambient temperature equilibrium Step 12: Minimize pipette handling Questions : How important is it to transfer the proper liquids in their proper containers? What will happen if you placed the wrong volume of liquids?

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