Methods 1 GIow cels for seeral generations in medium that contains amino acids [2 Transferthe cells to a medium that contains amino acids labeled with a light isotope. labeled with a heavy isotope. Change medium Replication Isolate 3 Isolate histone ctamers before and after replication... Isolate octamers octamers 4..and subject them to dersity gradient centrifugation. Spin Spin 5 Newly synthesied octamers are less dense and thus will be Results higher in the tube. 6od octamers are dense and Single band; old octamers Broad band; octamers with mixture of old and new histones will move toward the bottom of the tube. with heavy amino acids (heavy and light amino acids)
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
What results would be expected in the experiment outlined in Figure if, during replication, all the original histone proteins remained on one strand of the DNA and new histones attached to the other strand?
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