i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity %3D %3D E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ none +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Arial
BIUA
11
+
.. | I 1 I
3 I 4
i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity
E. coli chromosome
F' Plasmid
B-gal activity?
Permease activity?
When
When
When
When
Glucose is
Lactose is
Glucose is
Lactose is
present
present
present
present
a.) I+ P+ O+ Z+ Y+
Inone
+++
+++
b.) I^[S] P+ O+ Z+ Y+
none
c.) I+ P+ O^[c] Z+ Y+
none
d.) I+ P+ O- Z- Y+
none
e.) I+ P+ O+ Z+ Y+
I^[S] P+ O+ Z+ Y+
f.) I^[S] P+ O+ Z- Y+
I+ P+ O^[c] Z+ Y-
g.) I^[TB] P+ O+ Z+ Y
1+ P+ O^[c] Z- Y+
h.) I+ P+ O^[c] Z+ Y-
I+ P+ O+ Z** Y+
i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+
Z** is a polar mutation
ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it
nonfunctional, how would that affect the level of ß-galactosidase protein activity with
and without lactose present, why?
MacBook Air
000
Transcribed Image Text:Arial BIUA 11 + .. | I 1 I 3 I 4 i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ Inone +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why? MacBook Air 000
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