Many enzymes are inhibited irreversibly by heavy metal ions such as Hg²+ , Cu²+ , or Ag*, which can react with essential sulfhydryl groups to form mercaptides. Enz-SH + Ag* → Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. To 10.0 mL of a solution containing 1.2 mg/mL of a pure enzyme, an investigator added just enough AGNO, to completely inactivate the enzyme. A total of 0.346 µmol AGNO, was required. Calculate the minimum molecular weight (M,) of the enzyme. minimum M, = The determined M, is not exact, but it is a minimum value for the M, of the enzyme. Why does the M, value obtained this way give only the minimum molecular weight? The Ag* would also react with the terminal carboxyl group. O There is only one polypeptide chain. There are no disulfide bonds in the protein. ) There is only one titratable –SH group per protein molecule.

Many enzymes are inhibited irreversibly by heavy metal ions such as Hg²+ , Cu²+ , or Ag, which can react with essential sulfhydryl groups to form mercaptides. Enz-SH + Ag → Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. To 10.0 mL of a solution containing 1.2 mg/mL of a pure enzyme, an investigator added just enough AGNO, to completely inactivate the enzyme. A total of 0.346 µmol AGNO, was required. Calculate the minimum molecular weight (M,) of the enzyme. minimum M, = The determined M, is not exact, but it is a minimum value for the M, of the enzyme. Why does the M, value obtained this way give only the minimum molecular weight? The Ag* would also react with the terminal carboxyl group. O There is only one polypeptide chain. There are no disulfide bonds in the protein. ) There is only one titratable –SH group per protein molecule.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Concept explainers

Catalysis and Enzymatic Reactions

Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.

Lock And Key Model

The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.

Question

Many enzymes are inhibited irreversibly by heavy metal ions such as Hg²+ , Cu²+ , or Ag*, which can react with essential
sulfhydryl groups to form mercaptides.
Enz-SH + Ag+ → Enz–S-Ag + H*
The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate –SH groups quantitatively. To 10.0 mL
of a solution containing 1.2 mg/mL of a pure enzyme, an investigator added just enough A£NO, to completely inactivate
the enzyme. A total of 0.346 µmol AgNO, was required. Calculate the minimum molecular weight (M,) of the enzyme.
minimum M, =
The determined M, is not exact, but it is a minimum value for the M, of the enzyme.
Why does the M, value obtained this way give only the minimum molecular weight?
The Ag* would also react with the terminal carboxyl group.
There is only one polypeptide chain.
There are no disulfide bonds in the protein.
O There is only one titratable –SH group per protein molecule.

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