An enzyme reaction produces 1.2 x 10-4 M hydrogen ions during the 1.0 min duration of the reaction. The enzyme reaction mixture is buffered by a 0.10 M Tricine (pKa 8.16), pH 8.3.  What is the new pH after the reaction is over?  *hint* the Henderson-Hasselbalch equation is used twice*

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An enzyme reaction produces 1.2 x 10-4 M hydrogen ions during the 1.0 min duration of the reaction. The enzyme reaction mixture is buffered by a 0.10 M Tricine (pKa 8.16), pH 8.3.  What is the new pH after the reaction is over?  *hint* the Henderson-Hasselbalch equation is used twice*

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