Knowing that liquid liquid phase separation will occur when the change of gibbs free energy of the system is negative and the gibbs free energy is contributed of enthalpy and entropy. Please states all the conditions (maybe like increasing/decreasing the concentration of that molecules? etc.) in biological system that induce liquid liquid phase separation.

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Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.