kDa 245- 180. 140 100. 75. 60- 50 Loading Control 20- Leptin 15 10- Healthy Obese Obese + TE1 Obese + TE2 (Hypothetical immunoblot data for instruction/educational purposes only) Q.16 Based on the hypothetical blot above, was proper loading observed? Q.17 Assign intensity values (1-10) for each of the conditions above, then convert that to a bar chart by comparing it relative to "healthy" and paste it below (meaning divide each values to "healthy" value. Assume each value is the average for 3 trials so you could add error bars in your plot, and label it properly. I

kDa 245- 180. 140 100. 75. 60- 50 Loading Control 20- Leptin 15 10- Healthy Obese Obese + TE1 Obese + TE2 (Hypothetical immunoblot data for instruction/educational purposes only) Q.16 Based on the hypothetical blot above, was proper loading observed? Q.17 Assign intensity values (1-10) for each of the conditions above, then convert that to a bar chart by comparing it relative to "healthy" and paste it below (meaning divide each values to "healthy" value. Assume each value is the average for 3 trials so you could add error bars in your plot, and label it properly. I

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter42: The Immune System

Section: Chapter Questions

Problem 22CTQ: Different MHC I molecules between donor and recipient cells can lead to rejection of a transplanted...

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2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?
How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..
Can S-layer proteins be detected by immunolabelling when a capsule is present? How do you know? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
Plasma IL-12, IL-17, IL-18 and IL-4 concentrations of 36 Systemic Lupus Erythematosus (SLE) patients were determined. Eighteen sex- and age-matched control subjects were included. The cytokine concentrations were determined by enzyme linked immunosorbent assay. The mean duration of the diagnosis of SLE at the time when patients were evaluated for this study was 5.9-6.8 years. The results are shown in Table 1. Table 1 Mean ±s.d. (pg/ml) Cytokine Patients Controls P value IL-12 166.7±84.5 93.5土39.2 <0.001 IL-17 76.5±45.7 37.6±35.3 0.002 IL-18 IL-4 368.7±199.5 27.1±15.3 141.1土47.1 17.3±7.2 <0.001 <0.05 Discuss the distribution of the cytokines shown in this study and relates them with the pathogenicity of SLE.
5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH MG132 (h) 0 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? ● P53 P27 AGR2 GAPDH b) How do we know that the differences we observe in the proteins is not due to un- equal loading of samples into each lane of the gel?
22:23 1O 000 · 11:24 A9 OB1 r ll l 52% . +964 782 734 3923 2m541139927815107... Patient Encounter Part 3 The pretreatment workup is summarized below. Pathology: 47-year-old female with new diagnosis of infiltrating intraductal adenocarcinoma involving the left breast and regional node. Further tests on tumor samples indicated ER (8%), PR (negative), HER2 (negative), Ki-67 (72%), and grade (poorly differentiated). Intrinsic subtype (luminal B, HER2-negative). Radiology: FDG-PET/CT indicated a 5.3 x 2.5 cm mass in the left breast which appeared to extend to the epidermis of the skin; one node in the left axilla was also involved with tumor. No other evidence of distant disease was visualized. Laboratory: CBC, liver, and kidney function tests WNL, alkaline phosphatase and calcium are normal also. Stage: IB (T, N, M,) List the most important prognostic factors in this patient with newly diagnosed breast cancer. Assess the patient's level of risk for relapse. 50 SECTION 16 | ONCOLOGIC…
BIO152L Worksheet for Howard Hughes Medical Institute: The Virtual Immunology Lab Name: Student ID number: Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? 6. In general, what is an ELISA test used to detect? 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis (The answers to the following question can be found in the “Diagnosis” tab) 8. What are the 3 important limitations of an ELISA? Explain each.…
There are many immunotherapeutics now being used to immunomodulate recipients that are either fully human monoclonal antibodies or partially humanized monoclonal antibodies. Discuss whether or not they may interfere with solid phase antibody assays, CDC assays or cell based flow cytometry crossmatches.
Rheumatoid Factor RF Latex Test RF is a rapid latex agglutination test kit for the detection of rheumatoid factor (RF) in human serum. RF is found in sera of patients with rheumatoid arthritis and is believed to be IgM antibodies directed against the patients own immunoglobulin G. Principle: The RF latex particles are coated with specially purified human gamma globulin. When the latex suspension is mixed with serum containing elevated RF levels on a slide, clear agglutination is seen within 2 minutes. Questions 1. Is Rheumatoid Arthritis an autoimmune disease? 2. Differentiate the different types of Immunoglobulins 3. Differentiate Rheumatoid Arthritis from Gouty Arthritis
AUTOIMMUNE DISEASE ASSAYS ANA (Anti-Nuclear Antibody) What other methods are used to test for presence of ANAs? Give the principle of each. How does each of the listed methods differ from the one performed in the lab?
AUTOIMMUNE DISEASE ASSAYS ANA (AntiNuclear Antibody) Why are ANAs produced by the body? How are these ANAs produced by the body? Why is the homogeneous pattern most common in SLE?
Explain the statistical findings regarding the lateral flowimmunochromatographic assays sensitivity and robustness when compared to other test methods? Please discuss refering to original articles as examples Covid 19.