Is the ubiquitination relatively stable or highly dynamic? how does ubiquitination become detached from the protein of interest? what enzyme is involved?
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
(b) Is the ubiquitination relatively stable or highly dynamic? how does ubiquitination become detached from the protein of interest? what enzyme is involved?
The translation is a process through which ribosomes synthesizes polypeptide chains using mRNA. This polypeptide chain then undergoes post-translational modification to form a mature protein. These modifications are generally covalent and enzymatic. Ubiquitinations is one of the post-translational modification.
Ubiquitination is an enzymatic and covalent post-translational change. In this process, the glycine residue of ubiquitin-protein gets attached to the lysine residue of the substrate by forming an isopeptide bond. The process of ubiquitination involves three steps activation, conjugation, and ligation for which ubiquitin-activating enzyme E1s, ubiquitin-conjugating enzyme E2s, and ubiquitin ligase enzyme E3s are required respectively. It regulated degradation of proteins, cellular localization, activation, deactivation, modulation of interactions between proteins. It is a highly dynamic process.
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