Int. (A.U.) Int. (A.U.) 200 150 100 50 1. Haloperidol 2. Triflouroperazine 3. Trihexyphenidyl ° 4.0 8.0 12.0 16.0 Time (min) HPLC separation of antipsychotics drugs. 1. Haloperidol 2. Triflouroperazine 3. Trihexyphenidyl 2 200 88 150 100 50 0 4.0 8.0 12.0 16.0 Time (min) SFC separation of antipsychotics drugs. H, mm 0.080 0.070 0.060 HPLC 0.050 0.040 0.030 0.020 0.010 0.000 4x- SFC 3x 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Average linear velocity, cm/s FIGURE 29-3 Performance characteristics of a 5-um octadecyl (C) bonded silica column when elution is carried out with a conven- tional mobile phase (HPLC) and supercritical carbon dioxide SFC. (Adapted from D. R. Gere, Science, 1983, 222, 253, DOI: 10.1126/ science.6414083.)
Int. (A.U.) Int. (A.U.) 200 150 100 50 1. Haloperidol 2. Triflouroperazine 3. Trihexyphenidyl ° 4.0 8.0 12.0 16.0 Time (min) HPLC separation of antipsychotics drugs. 1. Haloperidol 2. Triflouroperazine 3. Trihexyphenidyl 2 200 88 150 100 50 0 4.0 8.0 12.0 16.0 Time (min) SFC separation of antipsychotics drugs. H, mm 0.080 0.070 0.060 HPLC 0.050 0.040 0.030 0.020 0.010 0.000 4x- SFC 3x 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Average linear velocity, cm/s FIGURE 29-3 Performance characteristics of a 5-um octadecyl (C) bonded silica column when elution is carried out with a conven- tional mobile phase (HPLC) and supercritical carbon dioxide SFC. (Adapted from D. R. Gere, Science, 1983, 222, 253, DOI: 10.1126/ science.6414083.)
Chapter14: Chromatography
Section: Chapter Questions
Problem 9P
Related questions
Question
Compare these chromatograms of three anti-psychotic drugs done by HPLC and SFC.
Why is there the difference in separation time for SFC versus HPLC? Hint, use the Van Deemter plot as a guide in answering this question. Why, fundamentally, would you expect a faster separation for SFC than HPLC, in general?
Transcribed Image Text:Int. (A.U.)
Int. (A.U.)
200
150
100
50
1. Haloperidol
2. Triflouroperazine
3. Trihexyphenidyl
°
4.0
8.0
12.0
16.0
Time (min)
HPLC separation of antipsychotics drugs.
1. Haloperidol
2. Triflouroperazine
3. Trihexyphenidyl
2
200
88
150
100
50
0
4.0
8.0
12.0
16.0
Time (min)
SFC separation of antipsychotics drugs.
Transcribed Image Text:H, mm
0.080
0.070
0.060
HPLC
0.050
0.040
0.030
0.020
0.010
0.000
4x-
SFC
3x
0.2 0.4 0.6 0.8 1.0 1.2 1.4
Average linear velocity, cm/s
FIGURE 29-3 Performance characteristics of a 5-um octadecyl (C)
bonded silica column when elution is carried out with a conven-
tional mobile phase (HPLC) and supercritical carbon dioxide SFC.
(Adapted from D. R. Gere, Science, 1983, 222, 253, DOI: 10.1126/
science.6414083.)
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