Background Enzymes are biological catalysts that increase the rates of chemical reactions by lowering the activation energies for those reactions. Chymotrypsin is a major pancreatic enzyme, and is a member of the serine protease family. This family of enzymes catalyse the hydrolytic cleavage of peptide bonds. The major difference among members of the serine proteases is specificity. Chymotrypsin is specific for peptides of the amino acids phenylalanine, tyrosine and tryptophan. It attacks only peptide bonds on the carboxyl side of tyrosine, tryptophan and phenylalanine (hydrophobic or aromatic amino acids) (SEE LECTURE NOTES). Pre-practical "MUST DO" to be included in your lab-book: 1. Reaction mechanism 2. Draw the GPNA molecule and identify the phenylalanine portion. 3. Draw L-phenylalanine with the correct stereochemistry 4. Why do you need to add the substrate into the buffer in the cuvette and not the other way around? 5. Why do you need to add the enzyme just before starting UV acquisition? HO. GPNA NO 2 Chymotrypsin H2O HO. GP ΌΗ + NO2 NH2 NA

Background Enzymes are biological catalysts that increase the rates of chemical reactions by lowering the activation energies for those reactions. Chymotrypsin is a major pancreatic enzyme, and is a member of the serine protease family. This family of enzymes catalyse the hydrolytic cleavage of peptide bonds. The major difference among members of the serine proteases is specificity. Chymotrypsin is specific for peptides of the amino acids phenylalanine, tyrosine and tryptophan. It attacks only peptide bonds on the carboxyl side of tyrosine, tryptophan and phenylalanine (hydrophobic or aromatic amino acids) (SEE LECTURE NOTES). Pre-practical "MUST DO" to be included in your lab-book: 1. Reaction mechanism 2. Draw the GPNA molecule and identify the phenylalanine portion. 3. Draw L-phenylalanine with the correct stereochemistry 4. Why do you need to add the substrate into the buffer in the cuvette and not the other way around? 5. Why do you need to add the enzyme just before starting UV acquisition? HO. GPNA NO 2 Chymotrypsin H2O HO. GP ΌΗ + NO2 NH2 NA

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter31: Immunochemistry

Section: Chapter Questions

Problem 31.75P

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