In step 2 (above) in the identification of the colony that contains a targeted gene, ____.   a. bacterial cells are cloned   b. cells with the targeted gene are identified   c. bacterial colonies are transferred   d. DNA is lysed   e. bacterial cells are ruptured

Q: Unlike the virus natural replication - molecular testing of Sars-CoV-2 uses two enzymes in vitro (in…

A: The coronavirus virus, which causes coronavirus illness, 19 (COVID-19). The coronaviruses are a…

Q: The in situ hybridization method is based on Select one: a. labeling specific neuronal components…

A: Hybridization is the property of single-stranded nucleic acids to base-pair with their complementary…

Q: Gene therapy is performed by ____.​   a. ​replacing a defective enzyme in an individual with a…

A: Gene therapy is a biotechnique that uses the gene transfer mechanism to treat the defected or…

Q: the best technique to answer the following question: Does GAL4 transcription activator bind to GAL3…

A: We can detect GAL4 transcription activator binds to GAL3 co-repressor via Bacterial two-hybrid…

Q: In gene targeting, homologous recombination between thebacterial chromosome and a linear _______-…

A: Genetic engineering involves the process of using recombinant DNA (rDNA) technology to alter the…

Q: Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end…

A: Base editing  It is an alternative tool to HDR-mediated replacement. This facilitates editing of…

Q: In 1996, a group of scientists from Scotland used a somatic cell of an adult sheep and fused it with…

A: Cloning

Q: Choose the statement that best describes the outcome of transfecting cells with a plasmid that…

A: A plasmid is an extra chromosomal DNA present within a cell. It is physically separated from the…

Q: You are determined to produce an antiserum for the next pandemic that has hit the world. You have…

A: Genetic modification is the method of altering an organism's genetic makeup using recombinant DNA…

Q: Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts…

A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: graduate student wants to isolate cells from a patient and grow them perpetually in culture to study…

A: The correct answers are as follows- A. Telomerase C. MDM2  A graduate student wants to isolate cells…

Q: c. How would you insert the gene of interest into the vector? d. How would get the vector into the…

A: DNA cloning refers to the process in which the DNA fragment is isolated and inserted into the…

Q: In Figure 5-12,a. Which Hfr gene enters the recipient last? (Whichdiagram shows it actually…

A: Some bacteria will have F-plasmid integrated with the chromosomal DNA, they are known as…

Q: which sample shows the plasmid is fully digested

A: 50 bp ladder sequence : 500 bp 450 bp 400 bp 350 bp 300 bp 250 bp 200 bp 150 bp 100 bp 50 bp

Q: Chromosomal DNA fragments for molecular cloning are cut by _________, and inserted into molecular…

A: Answer - Option B - Restriction endonucleases; Ligase

Q: Which of the following approaches you can use to locate the relative positions of genes on…

A: Genes are located on chromosomes. These genes can not be targeted without using any specific gene…

Q: The picture below is the structure of a bacterial Nucleoid Associated Protein (NAP) associated with…

A: In bacterial cells, the genetic material is organized and compacted into a structure called the…

Q: A mutation that eliminated the wild type function of PRE would be most consistent with... Select one…

A: Viruses that infect bacteria are known as bacteriophages. Phages have two life cycles: the lytic…

Q: Considering the proclivity of bacteria to share genetic information, discuss the concerns behind…

A: Mainly antibiotics are implicated against bacterial infections. Such as in common strep throat the…

Q: Describe how, using mice, a researcher could carry out (1) organismalcloning, (2) production of ES…

A: Cloning is the method by which an organism is created by the cells derived from a parent organism.…

Q: In the replica-plating experiments of the Lederbergs, bacterialcolonies appeared at the same…

A: Replica-plating experiment of Lederberg involves culturing bacteria on a non-selective media. Then…

Q: A particular strain of λ (lambda) can lysogenize its E. coli host at 30°C, but not at 42°C. Could a…

A: Integrase is a very important enzyme for integration of phage into its host genome by site specific…

Q: . In two isolates (one is resistant to ampicillin and theother is sensitive to ampicillin) of a new…

A: Bacteria are microscopic single-celled organisms that thrive in diverse environmental conditions.…

Q: a. be the only instance of that restriction site in the plasmid b. appear at least once in another…

A: A plasmid is a small circular double-stranded DNA molecule that differs from the chromosomal DNA of…

Q: 1. A set of 4 Hfr bacterial strains were used in a time-of-entry experiment to map the locations of…

A: Hfr strain is high frequency recombination cells. It is a bacteria  with conjugative plasmid . A…

Q: When bacteria are consumed by macrophages, the macrophages give off chemokines that recruit other…

A: Chemokines have many different activities, and in many cases, the microenvironment has a significant…

Q: following statements best describes the function of SDS in western blotting?

A: Western blotting is a technique used in molecule research studies to study a specific sequence of…

Q: used to confirm presence of 0.9 kb . dna sequencing  Pcr microarray a and b

A: A microarray is a laboratory tool for simultaneously detecting the expression of thousands of genes.…

Q: Microarrays a. must be preceded by a sothern blot b. compare gene expression levels across many…

A: Here's why the other options are incorrect:a. must be preceded by a southern blot: Southern blots…

Q: Read the statements below and determine which f the recombination mechanisms that it matches with -…

A: StatementTransformationConjugation (F+xF-)Conjugation (Hfr x F-)Generalized…

Q: The blotting technique used toidentify the isolated protein is ________A. Northern blottingB.…

A: Proteins are the type of macromolecules that are synthesized in the cytoplasm during the process of…