In spectrophotometry, we measure the concentration of an analyte by its absorbance of light. A low concentration sample was prepared, and nine replicate measurements gave absorbances of 0.0047, 0.0054, 0.0062, 0.0060, 0.0046, 0.0056, 0.0052, 0.0044, 0.0058.  Nine reagent blanks gave values of 0.0006, 0.0012, 0.0022, 0.0005, 0.0016, 0.0008, 0.0017, 0.0010, and 0.0011. a) What is the absorbance detection limit? This is related to both the scatter of the blank and the scatter in the measured samples. b) The calibration curve is a graph of absorbance versus concentration. Absorbance is a dimensionless quantity. The slope of the calibration curve is m = 2.24 x 104 M-1. Find the concentration detection limit. c) Find the lower limit of quantitation.

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Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.