In spectrophotometry, we measure the concentration of an analyte by its absorbance of light. A low concentration sample was prepared, and nine replicate measurements gave absorbances of 0.0047, 0.0054, 0.0062, 0.0060, 0.0046, 0.0056, 0.0052, 0.0044, 0.0058. Nine reagent blanks gave values of 0.0006, 0.0012, 0.0022, 0.0005, 0.0016, 0.0008, 0.0017, 0.0010, and 0.0011. a) What is the absorbance detection limit? This is related to both the scatter of the blank and the scatter in the measured samples. b) The calibration curve is a graph of absorbance versus concentration. Absorbance is a dimensionless quantity. The slope of the calibration curve is m = 2.24 x 104 M-1. Find the concentration detection limit. c) Find the lower limit of quantitation.

In spectrophotometry, we measure the concentration of an analyte by its absorbance of light. A low concentration sample was prepared, and nine replicate measurements gave absorbances of 0.0047, 0.0054, 0.0062, 0.0060, 0.0046, 0.0056, 0.0052, 0.0044, 0.0058. Nine reagent blanks gave values of 0.0006, 0.0012, 0.0022, 0.0005, 0.0016, 0.0008, 0.0017, 0.0010, and 0.0011. a) What is the absorbance detection limit? This is related to both the scatter of the blank and the scatter in the measured samples. b) The calibration curve is a graph of absorbance versus concentration. Absorbance is a dimensionless quantity. The slope of the calibration curve is m = 2.24 x 104 M-1. Find the concentration detection limit. c) Find the lower limit of quantitation.

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter36: Bone Densitometry

Section: Chapter Questions

Problem 4RQ

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In spectrophotometry, we measure the concentration of an analyte by its absorbance of light. A low concentration sample was prepared, and nine replicate measurements gave absorbances of: 0.0047, 0.0054, 0.0062, 0.0060, 0.0046, 0.0056, 0.0052, 0.0044, 0.0058. Nine reagent blanks gave values of: 0.0006, 0.0012, 0.0022, 0.0005, 0.0016, 0.0008, 0.0017, 0.0010, and 0.0011. a) What is the absorbance detection limit? This is related to both the scatter of the blank and the scatter in the measured samples.
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