In eukaryotic cells, a poly(A) tail is normally added to pre-mRNA, but not to rRNA or tRNA. With the use of recombinant DNA techniques, a protein-encoding gene (which is normally transcribed by RNA polymerase II) can be connected to a promoter for RNA polymerase I. This hybrid gene is subsequently transcribed by RNA polymerase I, and the appropriate pre-mRNA is produced, but this pre-mRNA is not cleaved at the 3′ end, and a poly(A) tail is not added. Propose a mechanism to explain how the type of promoter found at the 5′ end of a gene can affect whether a poly(A) tail is added to the 3′ end.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
In eukaryotic cells, a poly(A) tail is normally added to pre-mRNA, but not to rRNA or tRNA. With the use of recombinant DNA techniques, a protein-encoding gene (which is normally transcribed by RNA polymerase II) can be connected to a promoter for RNA polymerase I. This hybrid gene is subsequently transcribed by RNA polymerase I, and the appropriate pre-mRNA is produced, but this pre-mRNA is not cleaved at the 3′ end, and a poly(A) tail is not added. Propose a mechanism to explain how the type of promoter found at the 5′ end of a gene can affect whether a poly(A) tail is added to the 3′ end.
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