When Laybourne and Kadonaga studied the effects of histone proteins on eukaryotic transcription using an in vitro transcription assay explain why: a) they used two different DNA templates that contained different promoter structures. b) when they included both activator protein and histones, they always added the histone protein before adding the activator to the transcription assay mixture. (Ctri) -

Q: Eukaryotic transcription requires a "cap" at one end and a "tail" at the other end of each mRNA…

A: Eukaryotic transcription is the process that eukaryotic cells use to copy genetic material through…

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: How far apart are transcription bubbles on E. coli genes that are being transcribed at a maximal…

A: The transcription bubble is an area of unpaired bases on one of the uncovered DNA strands. The…

Q: what is the highly compacted strongly staining region of the chromosome with no transcriptional…

A: The highly compacted strongly region of the chromosome with no transcriptional activity is…

Q: Compare and contrast two mechanisms for transcriptional termination in bacteria.

A: The process of formation of the RNA strands from the DNA template by the action of an RNA polymerase…

Q: For the following gene, which type of regulatory sequence has likely been deleted in mutant 1?…

A: Transcription is the mechanism by which an RNA copy of a gene sequence is produced. This clone,…

Q: You construct a translational reporter gene fusion of your favourite gene. How can you assess…

A: Translational Gene Fusion is employed to live protein localization by quantifying promoter activity.…

Q: During eukaryote transcription initiation, when TFIIE, TFIIA, TFIIB, TFIIF, and RNA pol II all join…

A: Transcription factor is a protein that binds to a specific region in DNA that controls the…

Q: control medium that demonstrates the general growth rate of mutant candidates

A: Control Medium: This medium is used to demonstrate the general growth rate of mutant candidates in…

Q: A rice MADS box transcription factor gene contains a number of sequences that are removed in the…

A: In eukaryotes including rice mRNA processing involves the removal of introns which are non-coding…

Q: Suppose you want to study the transcription in vitro of one particular gene in a DNA molecule that…

A: Transcription is the first step in gene expression during which the nucleotide sequence of DNA is…

Q: Please describe the procedures of initiation during transcription in bacteria.

A: Elongation of transcription begins after initiation where the dinucleotides are added to the mRNA…

Q: The presence of a DNA template (e.g. a product from PCR), general transcription factors, and RNA…

A: To answer this question, let us first address the main difference between genomic DNA and the PCR…

Q: The IMD2 promoter contains three upstream transcription start sites (TSS) that are utilized under…

A: Inosine monophosphate dehydrogenase (IMD2) is a key enzyme in the purine biosynthesis pathway that…

Q: Consider a gene being transcribed at a constant rate k1 and being degraded with first  order…

A: The genetic information from protein synthesis is stored in DNA. This information is copied onto an…

Q: Explain how a transcriptional fusion to a reporter gene, combined with site directed mutagenesis,…

A: Studies of gene expression and promoter activity are frequently made easier with the aid of gene…

Q: 7) Which of the following needs to occur for transcription to take place in prokaryotes? A) The RNA…

A: Prokaryotic transcription is the process by which the genetic material in prokaryotes is converted…

Q: Once an RNA polymerase has initiated transcription, it will release the sigma factor or sigma…

A: TRANSCRIPTION- The process of formation of mRNA from the DNA strand is known as transcription. It is…

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.