In cyclic photophosphorylation, it is estimated that two electrons must be passed through the cycle to pump enough protons to generate one ATP. Assuming that the AG for hydrolysis of ATP under conditions existing in the chloroplast is about –50 kJ/mol, what is the corresponding percent ef- ficiency of cyclic photophosphorylation, using light of 700 nm?
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- In cyclic photophosphorylation, it is estimated that two electrons must be passed through the cycle to pump enough protons to gener- ate one ATP. Assuming that the AG for hydrolysis of ATP under con- ditions existing in the chloroplast is about –50 kJ/mol, what is the corresponding percent efficiency of cyclic photophosphorylation, using light of 700 nm?In cyclic photophosphorylation, it is estimated that two electrons must bepassed through the cycle to pump enough protons to generate one ATP.Assuming that the ∆G for hydrolysis of ATP under conditions existing inthe chloroplast is about -50 kJ/mol, what is the corresponding percent efficiency of cyclic photophosphorylation, using light of 700 nm?Cyclic photophosphorylation uses two photons to generate one ATP. If the AG of ATP hydrolysis is -50 kj/mol. What is the percent efficiency of cyclic photophosphorylation? F =96.5 kj/mol*V. Use c AG = -nFAV ⒸShow Transcribed Text to solve.
- Calculate (in kJ/mol) the energy that is required by the Rhodopseudomonas viridis photophosphorylation system to synthesize 3 ATP? (Assume that the R. viridis F1F-ATP synthase c-subunit rotor contains 12 c-subunits and that the R. viridis cytochrome b/c complex translocates 2 H/e". Also assume that the wavelength of lig! is 680 nm.) Number of photons required to synthesize 3 ATP = photons Energy = kJ/molFor the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the protons from the photosystems are transported, like with the mitochondrial inner membrane, against the gradient from the lumen to the stroma. However, the pH(lumen) – pH (stroma) is much larger, about 3.4. Calculate delta psi (the electric potential) for the thylakoid membrane, given that ∆G of proton transport is about the same as for the mitochondrion. Then comment on its permeability to ions, compared to the mitochondrial inner membrane’s permeability to ions.When running cycle photophosphorylation and passaging electrons from PSI to two fully reduced PQH2s, how many photons would be necessary for the electrons to complete one cycle?
- It is believed that the ratio of cyclic photophosphorylation to noncyclicphotophosphorylation changes in response to metabolic demands. Ineach of the following situations, would you expect the ratio to increase,decrease, or remain unchanged?(a) Chloroplasts carrying out both the Calvin cycle and the reduction ofnitrite (NO2-) to ammonia (This process does not require ATP.)(b) Chloroplasts carrying out not only the Calvin cycle but also extensiveactive transport(c) Chloroplasts using both the Calvin cycle and the C4 pathwayIdentify the chemical basis for ApH and AY across the chloroplast thylakoid membrane by dragging the descriptions to their targets. Be sure to notice that the upper arrow iindicates ApH and the lower arrow indicates ΔΨ. ATP synthase complex H+ N ADP + P₁ Light energy ATP H*N Photosystem I/II- Chloroplast N side Aus PN ApH T + Thylakoid membrane HTp H+p Lumen Stroma P side Proton circuit A B High H concentration Low positive charge High positive charge Low H+ concentration Within the image, identify the types of proton translocation by dragging each label to its target. O XH₂ 2H+ + Z 2 H* ZH₂ O XH₂ Z 2H+ ZH₂ 2H+ C A B Proton pump Redox loopWhat is photophosphorylation by oxidative phosphorylation? Explain by comparison.
- Thylakoids were isolated from chloroplasts and incubated in the dark in an acidic solution (pH 4) to equilibrate the pH. After 30 minutes, the thylakoids were transferred to a basic solution (pH 8) and kept in the dark. Will this system produce ATP? Explain. Will this system produce G3P? Explain.Assume a pH gradient of 4.0 units across a thylakoid membrane, with the lumen more acidic than the stroma.What is the standard free energy change per mol O2 produced? How does this compare to the energy required to drive the synthesis of ATP?The coenzyme NADP is the terminal electron acceptor in chloroplasts, according to the reaction 2 H₂O + 2 NADP+ 2 NADPH + 2 H+ + O₂ Calculate the equilibrium constant, K'eq, for this reaction at 25 °C. + Use an E'° of -0.324 V for NADP and 0.816 V for H₂O. K'eq = x10