Why is a hypotonic buffer used for the resuspension? 1.DCPIP passes easily across the intact chloroplast membrane, but can't get into the thylakoid, so electron transport could not be reliably measured if using a hypotonic buffer. 2.To gently lyse the chloroplasts, leaving the electron transport membranes intact, whilst allowing DCPIP access 3.To gently separate the chloroplasts from each other, leaving the electron transport membranes intact, whilst allowing DCPIP access 4.DCPIP cannot easily across the intact chloroplast membrane, so electron transport could not be reliably measured if using a hypotonic buffer.

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Photosynthesis

Plants have a capacity that we humans can only imagine— the ability to produce food from sunlight. Sure, we can prepare some dishes that we can savor on, but it become possible because of the plants (as they serve as a producers of all the raw materials required). Photosynthesis is the method of assembling carbohydrates with the aid of sunlight.