For the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the protons from the photosystems are transported, like with the mitochondrial inner membrane, against the gradient from the lumen to the stroma. However, the pH(lumen) – pH (stroma) is much larger, about 3.4. Calculate delta psi (the electric potential) for the thylakoid membrane, given that ∆G of proton transport is about the same as for the mitochondrion. Then comment on its permeability to ions, compared to the mitochondrial inner membrane’s permeability to ions.
For the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the protons from the photosystems are transported, like with the mitochondrial inner membrane, against the gradient from the lumen to the stroma. However, the pH(lumen) – pH (stroma) is much larger, about 3.4. Calculate delta psi (the electric potential) for the thylakoid membrane, given that ∆G of proton transport is about the same as for the mitochondrion. Then comment on its permeability to ions, compared to the mitochondrial inner membrane’s permeability to ions.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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For the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the protons from the photosystems are transported, like with the mitochondrial inner membrane, against the gradient from the lumen to the stroma. However, the pH(lumen) – pH (stroma) is much larger, about 3.4. Calculate delta psi (the electric potential) for the thylakoid membrane, given that ∆G of proton transport is about the same as for the mitochondrion. Then comment on its permeability to ions, compared to the mitochondrial inner membrane’s permeability to ions.
![Objective: Perform calculations about the free energy change and electric potential
across various membranes, illustrating differences between them.
1. Recall the membrane proton-transport free energy equation:
AG = 2.3 RT [pH(side 1) - pH (side 2)] + Z FAY
=
where Z = the charge on the proton (+1), F = Faraday's constant = 96485 C/mol and R
gas constant = 8.314 J/K mol.
For the inner membrane of a mitochondrion, AY = + 0.168 V; use T = 310K (37°C) and
pH(matrix) - pH (intermembrane space) = 0.75.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0627210d-c0e3-4a2a-8541-a7399a2e5e96%2F689a979f-128d-4877-98a1-e9dca5e2cef6%2Fjevfbt_processed.png&w=3840&q=75)
Transcribed Image Text:Objective: Perform calculations about the free energy change and electric potential
across various membranes, illustrating differences between them.
1. Recall the membrane proton-transport free energy equation:
AG = 2.3 RT [pH(side 1) - pH (side 2)] + Z FAY
=
where Z = the charge on the proton (+1), F = Faraday's constant = 96485 C/mol and R
gas constant = 8.314 J/K mol.
For the inner membrane of a mitochondrion, AY = + 0.168 V; use T = 310K (37°C) and
pH(matrix) - pH (intermembrane space) = 0.75.
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