Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are luciferin+O2 ⇌ oxyluciferin+light ATP ⇌ AMP+PPi, ΔGo=−31.6 kJ/mol If the overall ΔGo of the coupled reaction is -8.70 kJ/mol, what is the equilibrium constant, K, of the first reaction at 27oC?

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