In a molecular biology laboratory, a student obtained competent E. coli cells and used a common transformation procedure to induce the uptake of plasmid DNA with a gene for resistance to the antibiotic kanamycin. The results below were obtained. Plate I. LB agar kan plasmid Plate II. LB agar with kanamycin kan plasmid Plate III. LB agar no plasmid added Plate IV. LB agar with kanamycin no plasmid added On which petri dish do only transformed cells grow? b. Plate Il a. Plate I c. Plate II d. Plate IV Which of the plates is used as a control to show that non-transformed E. coli will not grow in the presence of kanamycin? a. Plate | b. Plate II C. Plate III d. Plate IV If a student wants to verify that transformation has occurred, which of the following procedures should s/he use? a. Spread cells from Plate I onto a plate with LB agar, incubate. b. Spread cells from Plate Il onto a plate with LB agar, incubate. c. Repeat the initial spread of-kank cells onto plate IV to eliminate possible experimental error. d. Spread cells from Plate Il onto a plate with LB agar with kanamycin; incubate. e. Spread cells from Plate III onto a plate with LB agar and also onto a plate with LB agar with kanamycin; incubate.
In a molecular biology laboratory, a student obtained competent E. coli cells and used a common transformation procedure to induce the uptake of plasmid DNA with a gene for resistance to the antibiotic kanamycin. The results below were obtained. Plate I. LB agar kan plasmid Plate II. LB agar with kanamycin kan plasmid Plate III. LB agar no plasmid added Plate IV. LB agar with kanamycin no plasmid added On which petri dish do only transformed cells grow? b. Plate Il a. Plate I c. Plate II d. Plate IV Which of the plates is used as a control to show that non-transformed E. coli will not grow in the presence of kanamycin? a. Plate | b. Plate II C. Plate III d. Plate IV If a student wants to verify that transformation has occurred, which of the following procedures should s/he use? a. Spread cells from Plate I onto a plate with LB agar, incubate. b. Spread cells from Plate Il onto a plate with LB agar, incubate. c. Repeat the initial spread of-kank cells onto plate IV to eliminate possible experimental error. d. Spread cells from Plate Il onto a plate with LB agar with kanamycin; incubate. e. Spread cells from Plate III onto a plate with LB agar and also onto a plate with LB agar with kanamycin; incubate.
Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Transcribed Image Text:In a molecular biology laboratory, a student obtained competent E. coli cells and used a common transformation
procedure to induce the uptake of plasmid DNA with a gene for resistance to the antibiotic kanamycin. The
results below were obtained.
Plate I.
LB agar
+kan plasmid
Plate II.
LB agar with kanamycin
+kan plasmid
Plate IV.
Plate II.
LB agar
no plasmid added
LB agar with kanamycin
no plasmid added
On which petri dish do only transformed cells grow?
b. Plate I|
a. Plate I
c. Plate III
d. Plate IV
Which of the plates is used as a control to show that non-transformed E. coli will not grow in the presence of
kanamycin?
a. Plate I
b. Plate II
c. Plate II
d. Plate IV
If a student wants to verify that transformation has occurred, which of the following procedures should s/he use?
a. Spread cells from Plate I onto a plate with LB agar, incubate.
b. Spread cells from Plate Il onto a plate with LB agar, incubate.
c. Repeat the initial spread of-kanR cells onto plate IV to eliminate possible experimental error.
d. Spread cells from Plate Il onto a plate with LB agar with kanamycin; incubate.
e. Spread cells from Plate III onto a plate with LB agar and also onto a plate with LB agar with kanamycin; incubate.
Transcribed Image Text:During the course of an E. coli transformation laboratory, a student forgot to mark the culture tube that
received the kanamycin-resistant plasmids. The student proceeds with the laboratory because he thinks that he
will be able to determine from his results which culture tube contained cells that may have undergone
transformation. Which plate would be most likely to indicate transformed cells?
a. A plate with a lawn of cells growing on LB agar with kanamycin
b. A plate with a lawn of cells growing on LB agar without kanamycin
c. A plate with 100 colonies growing on LB agar with kanamycin
d. A plate with 100 colonies growing on LB agar without kanamycin
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