In a FRET experiment designed to monitor conformational changes in T4 lysozyme, the fluorescence intensity fluctuates between 5000 and 10,000 counts per second. Assuming that 7500 counts represents a FRET efficiency of 0.6, what is the change in FRET pair separation distance during the reaction? For the tetramethylrhodamine-texas red FRET pair employed ro = 50. Å. Express your answer to two significant figures and include the appropriate units. HẢ ? Ar = Value Units

In a FRET experiment designed to monitor conformational changes in T4 lysozyme, the fluorescence intensity fluctuates between 5000 and 10,000 counts per second. Assuming that 7500 counts represents a FRET efficiency of 0.6, what is the change in FRET pair separation distance during the reaction? For the tetramethylrhodamine-texas red FRET pair employed ro = 50. Å. Express your answer to two significant figures and include the appropriate units. HẢ ? Ar = Value Units

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

In a FRET experiment designed to monitor conformational changes in T4 lysozyme, the fluorescence intensity fluctuates between 5000 and 10,000 counts per second. Assuming
that 7500 counts represents a FRET efficiency of 0.6, what is the change in FRET pair separation distance during the reaction? For the tetramethylrhodamine-texas red FRET
pair employed ro =
50. Å.
Express your answer to two significant figures and include the appropriate units.
HÀ
?
Ar =
Value
Units

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