In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 5 cm away from the finish line. The distance travelled from the starting line to finish line is 30 cm. The first amino acid, AA1 travelled 4 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 4 cm ahead of AA2. a) Reproduce the chromatogram. Label. b) Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
- In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 5 cm away from the finish line. The distance travelled from the starting line to finish line is 30 cm. The first amino acid, AA1 travelled 4 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 4 cm ahead of AA2.
a) Reproduce the chromatogram. Label.
b) Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____
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