what does it mean if a pigment (let' say 'yellow') traveled beyond the solvent front in paper chromatography
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Q: in paper chromatography what is the basis of pigments separate?
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what does it mean if a pigment (let' say 'yellow') traveled beyond the solvent front in paper chromatography?
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- in paper chromatography what is the basis of pigments separate?What is the basic purpose of the paper chromatography?Why is it important to select immiscible solvents when performing liquid-liquid extraction? In general, how can you determine which layer (aqueous or organic) is on top, and which is on the bottom?
- Chlorophyll is the green pigment in the leaves of the plant, carotene imparts orange coloration while anthocyanin is known as the purple pigment. In one of their laboratory, a group was tasked to confirm the presence of these pigments on a certain leaf. After crushing several leaves, they soak it in isopropyl paper strip was dried and the group got the following measurements from the submerged tip of the paper strip: solvent font - 9.9cm, purple ink -8.6cm, yellowish orange ink - 6.4cm and green ink - 4.2 retention factor of each pigment. (d) Rank each pigment according to their solubility in the given solvent.NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's insensitivity?What is the working principle of raman spectroscopy.
- Based on the data chart below, how far did the solvent travel up the chromatography paper?Three sugars (Sugar A, B and C) were applied to a line as part of the set-up of a Paper Chromatography experiment (method was as described in Practical Manual. After running the chromatography and staining, the following Retention factor (Rf) values were determined. Note that the distance travelled by the solvent was equal across the width of the chromatography paper. Sugar A, Rf = 0.23 Sugar B, Rf = 0.65 Sugar C, Rf = 0.27 What can be drawn from the results of this Paper Chromatography experiment? O Sugars A and C had a stronger interaction with the mobile phase, relative to Sugar B. O Sugar B had the strongest interaction with the stationary phase among Sugars A, B, and C. O Sugar B had the strongest interaction with the mobile phase among Sugars A, B, and C. O Distance travelled by Sugar B must have been shorter than that of Sugars A and C. O Sugar B must be a non-reducing sugar given its high Rf value.why steroids do not show luminescence on thin layer chromatographic plates?