Instructions for washing your crystallized product in the vacuum filter funnel call for using the ice-cold wash solvent. Why is this importan

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Instructions for washing your crystallized product in the vacuum filter funnel call for using the ice-cold wash solvent. Why is this important? How would the success of the experiment be affected if the wash solvent were warm?

   

The purity of the substance would decrease with a warm wash.

   

The solubility of the substance would decrease with a warm solvent. 

   

More of the product will dissolve if the wash step is carried out with warm solvent. 

   

Warm solvent does not filter as well as cold solvent. 

   

The solubility of impurities would decrease with a warm solvent. 

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