When deoxyhemoglobin was first isolated in crystalline form, the researcher who did so noted that the crystals changed color from purple to red and also changed shape as he observed them under a microscope. What is happening on the molecular level? Hint: The crystals were mounted on a microscope slide with a loosely fitting cover slip.

Q: Consider analysis of a mixture of saturated and unsaturated triacylglycerols (fish oil) and/or fatty…

A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbon to 36 carbons. They…

Q: In some autoimmune diseases, an individual develops antibodies that recognize cell constituents such…

A:

Q: Which nuclear isotope used in protein NMR spectroscopy is the most sensitive to detect? Briefly…

A: NMR Spectroscopy is used to study the structure and composition of biomolecules like proteins. Here…

Q: In an anomeric pairs of monosaccharides, the b form has the —OH below the ring. A) True B) False

A: Monosaccharides are simple form of sugars such as glucose.

Q: Draw a structure for the compound, C3H5Br, that fits the following 1H NMR data: δ 2.32 (3H, singlet)…

A: Nuclear magnetic resonance Spectrum represents the different interactions of stereochemically…

Q: Explain why it has been very difficult to obtain an X-ray crystal structure for the complete nuclear…

A: A substantial and intricate protein structure called the nuclear pore complex (NPC) covers the…

Q: The following stock solutions are available to make a protein extraction buffer: 100% Nonidet P-40,…

A: Molar concentration, also known as molarity, it is a number of moles of solute in a solution. Molar…

Q: Some characteristics of three proteins are listed in the table below: Protein Molecular Weight (Da)…

A: Gel filtration chromatographic procedure in which the components are separated according to their…

Q: 1) A ligand-binding protein showing negative homotropic cooperativity? a) should give an nH value…

A: Cooperativity or cooperative binding occurs when binding of one molecule influences the affinity of…

Q: A nonapeptide was treated with dithiothreitol to reduce any disulfide bridges, then partially…

A: Amino acids are the basic unit of the peptide and proteins. They are joined via the peptide bonds to…

Q: A protein was purified to homogeneity. Determination of the mass by gel-filtration chromatography…

A: To homogeneity, the protein is purified.  When the determination of mass is done by the gel…

Q: Define a chaotropic agent.  What effect would a chaotropic agent have on protein structure?

A: Introduction :- Chaotropic agents are cosolutes that have the potential to damage the network of…

Q: A) Draw L-histidine. Label all ionizable groups with the appropriate pKa.

A: Introduction: Histidine is one of the 20 amino acids with imidazole ring that is highly basic by…

Q: For each of the following amino acids: 1) describe what the important functional group of the amino…

A: Proteins are made up of amino acids. Each amino acid has a distinct side chain, also referred to as…

Q: What is the function of the structure labeled A?

A: Annelids are bilaterally symmetrical(can be divided into two equal segmants), triploblast(have three…

Q: Of the components in the hydrogels beads, which is the protein we are interested in measuring?

A: Hydrogel beads are cross-linked mesh of hydrophilic polymers which is composed of spherical shaped…

Q: ou have given two proteins with a pI of 4.5 and pI of 7.7? Using an anion-exchange column, how can…

A: Ion-exchange chromatography: The method involves the separation of ions and polar molecules based…

Q: A protein contains three 60-kD polypeptides and six 20-kD polypeptides. Each 60-kD chain is…

A: Electrophoresis means migration of charged particles under the influence of an electric…

Q: Can the iodine test distinguish between amylose and amylopectin?  What would you look for?

A: Carbohydrates are an essential source of energy. It is found in almost all foods and is largely used…

Q: In Experiment 7, Isolation of Citrus Oils, the compounds isolated were the monoterpene compounds…

A: Chromatographic techniques are commonly used in chemistry and biochemistry to separate molecules…

Q: The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A…

A: Ion Exchange Chromatography (IEC) is a chromatography technique whose ability to separate analytes…

Q: what is the dna concentration suspended in PO4 buffer at ph 7.0 that has an absorbance at 260 nm…

A: DNA concentration of a sample can be determined using spectrophotometry and measuring the absorbance…

Q: Identify the following non-chiral molecule:  NH2-CH2-COOH (dry form) aspartic acid arginine glycine…

A: Amino acids are small molecules made up of carbon, hydrogen, nitrogen and oxygen in some cases…

Q: Using mass spec, the primary structure of 4 peptides is obtained from a tissue sample: VMDEL, GHKRT,…

A: Amino acid is the basic unit of a protein. An amino acid contains carboxylic acid groups (—COOH),…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper…

A: Paper chromatography is the analytical process by which mixtures of complex compounds such as amino…