MCQ.
It is possible to incubate your tissue with two different primary antibodies simultaneously. If this is done, it is imperative that you can track each primary antibody with a different color. For example, one secondary antibody is tagged with Alexa488 which emits as green and another secondary antibody is tagged with Alexa 555 which emits as orange. Which of the following immunohistochemistry protocols is appropriate?
a. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 secondary antibody
b. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 488 and anti-chicken Alexa 555 secondary antibodies
c. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 555 and anti-chicken Alexa 555 secondary antibodies
d. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 and anti-rabbit Alexa 555 secondary antibodies
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