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- A paste of starch was tested using Benedict’s test, the Iodine Test, and Fehling’s Test. Before hydrolysis, Benedict’s test and Fehling's test results were both negative while Iodine’s test is positive. After adding 0.5 mL of conc. HCl to the paste, another 5 drops of NaOH were added and was tested using Fehling’s, Benedict’s Test, and Iodine Tests. What would be the theoretical result of the three different tests? Explain the chemical reaction that happened and the principle/mechanism behind the reactions.Explain how acetaminopen gives such spectroscopic and chromatographic graph by interpreting the peaks that appeared in the graphs. Fig1. Mass Spectrometric Chromatogram of Acetaminophen (the two graphs) Fig2. IR Spectrograph of AcetaminophenA certain amount of white solid was dissolved in enough water and gives a colorless solution. A portion of the solution was added with 15% solution of alpha-naphthol solution and a few drops of conc. H2SO4 forming a violet-colored ring. Another portion was added with drops of Seliwanoff’s reagent and showed no change after being warmed in a bath. Another portion was added with equal amounts of Fehling’s A and B reagent giving a red precipitate in a blue solution after warming in a bath. a) Cite an experimental result confirming that the solid is a carbohydrate b) Is the solid starch, fructose or glucose? Support your answer.
- You have prepared the following serial dilution using tryptone broth:d1: 0.1ml culture + 9.9 ml brothd2: 300µl d1 + 4.7 ml brothd3: 0.2ml d2 + 9800 µl broth1) Write your dilutions as a rational number AND in scientific format? What was your total dilution (in scientific notation)?2) What was the original concentration of your culture if you spread 0.1 ml of d3 and counted360 cfu?The hydrolysis of starch was stopped when the iodine test no longer gave a blue color. Does this mean that the starch was completely hydrolyzed? Explain.The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?
- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.A student carries out the following experiments and records the following observations. 1. A few drops of a solution of KOH (aq) is added to a solution of Fe(NO3)3 (aq). A red gelatinous solid forms immediately upon addition. 2. A few drops of a solution of KOH (aq) is added to a solution of NaNO3 (aq). No changes are observed. 3. A few drops of a solution of KOH (aq) is added to a solution that could contain Fe3+(aq) and/or Na+(aq) ions (the solution could contain both ions, just one ion, or neither ions). A red gelatinous solid forms immediately upon addition. Select all of the answers that are consistent with the data from those 3 experiments. Group of answer choices The unknown contains Fe3+(aq) and Na+(aq) The unknown contains Fe3+(aq) It is not possible to determine the presence of Na+(aq) from this experiment The unknown contains neither Fe3+(aq) or Na+(aq)What do you think would happen if 0.5M K+ was used first to elute the sample in an ion exchange chromatography experiment? Why?
- Cyclophosphamide, after reconstitution, has a concentration of 20 mg/mL. The patient, who weighs 67 kg and is 155 cm tall, is ordered cyclophosphamide in 250 mL NS. How many mL will be added to the bag?The antibiotic, tetracycline was serially diluted (2-fold dilutions), starting with tube #1 (100 µg/ml) and ending with tube #9 (tube #10 = Control). Tetracycline is a yellow powder that is freely soluble in water. To prepare the tetracycline solution, dissolve the powder in water, with heating, to make 50 mg/ml. How will you prepare the solutions for the two-fold dilution? Create a scheme on how to perform the serial dilution using the 50 mg/mL stock solution and 5 mL as final volume for each step.After performing the manual Albumin assay, you get the absorbance value of 0.205 for a 4.5 g/dL albumin standard and 0.114 for control A. What is the calculated value of the control? (just write a number with one decimal, the units will be mg/dL)