If you were to set up a PCR reaction (in vitro DNA synthesis) with a DNA template, primers,DNA polymerase, DATP, dGTP, dCTP, dTTP and a small amount of ddATP, what would be the result? DNA synthesis would happen normally. All DNA molecules produced would be the same length as the template. DNA synthesis might be terminated after the addition of any adenine base (at random). DNA molecules of many different lengths would be produced. DNA synthesis would be terminated after the first adenine base is added. All DNA molecules produced would the same length, shorter than the template.
If you were to set up a PCR reaction (in vitro DNA synthesis) with a DNA template, primers,DNA polymerase, DATP, dGTP, dCTP, dTTP and a small amount of ddATP, what would be the result? DNA synthesis would happen normally. All DNA molecules produced would be the same length as the template. DNA synthesis might be terminated after the addition of any adenine base (at random). DNA molecules of many different lengths would be produced. DNA synthesis would be terminated after the first adenine base is added. All DNA molecules produced would the same length, shorter than the template.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:If you were to set up a PCR reaction (in vitro DNA synthesis) with a DNA template, primers,DNA polymerase, DATP, dGTP, dCTP, dTTP and a small amount of
ddATP, what would be the result?
DNA synthesis would happen normally.
All DNA molecules produced would be the same length as the template.
DNA synthesis might be terminated after the addition of any adenine base (at random).
DNA molecules of many different lengths would be produced.
DNA synthesis would be terminated after the first adenine base is added.
All DNA molecules produced would the same length, shorter than the template.
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