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Write the nucleotide sequence, including the primer, in the 3'to 5' direction. 3'- -5'

Write the nucleotide sequence, including the primer, in the 3'to 5' direction. 3'- -5'

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A researcher sequences a DNA fragment using dye-terminator sequencing. In this procedure, each dideoxynucleotide triphosphate (ddNTP) contains a fluorescent marker specific to the base. Upon excitement by a laser, the ddNTPs emit light at their characteristic wavelengths. An automated machine then reads the sequence. In this procedure, • ddCTP is tagged with a blue marker that emits light at 517 nm, • ddATP is tagged with a green marker that emits light at 540 nm, • ddGTP is tagged with a yellow marker that emits light at 570 nm, and • ddTTP is tagged with a red marker that emits light at 635 nm. The primer has the sequence 5'-ATGAC-3'. In the image shown here, the shortest fragments are at the bottom and the longest fragments are at the top. C T A T с T T T с G G Write the nucleotide sequence, including the primer, in the 3' to 5' direction. 3'- -5'
Transcribed Image Text:A researcher sequences a DNA fragment using dye-terminator sequencing. In this procedure, each dideoxynucleotide triphosphate (ddNTP) contains a fluorescent marker specific to the base. Upon excitement by a laser, the ddNTPs emit light at their characteristic wavelengths. An automated machine then reads the sequence. In this procedure, • ddCTP is tagged with a blue marker that emits light at 517 nm, • ddATP is tagged with a green marker that emits light at 540 nm, • ddGTP is tagged with a yellow marker that emits light at 570 nm, and • ddTTP is tagged with a red marker that emits light at 635 nm. The primer has the sequence 5'-ATGAC-3'. In the image shown here, the shortest fragments are at the bottom and the longest fragments are at the top. C T A T с T T T с G G Write the nucleotide sequence, including the primer, in the 3' to 5' direction. 3'- -5'
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