How would you make wash buffer with 0.05M Tris containing 0.05M NaCl in 200ml at pH of 8.0

Q: prepare 250ml of 0.10 m NaOH from solid NaOH and water

A: Molarity: The moles of a solute per litre of a solution is called as molarity (M).

Q: How much of a 10x buffer should be added to 900ml of water to make a 1x solution?

A: In this question, we are given with- 1. Concentration of stock solution= 10X 2. Required…

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A: Introduction  When modest amounts of acid or base are given to a solution, the pH does not alter as…

Q: If I want to make a 100mL buffer that has 2% SDS, 100mM tris pH 8, 10mM EDTA, and proteinase K…

A: Introduction: Buffer is defined as a solution that resists change in pH on the addition of acid or…

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A: Buffer solutions are solutions that resist change in pH . Biochemical reactions in the laboratory…

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Q: The following stock solutions are available to make a protein extraction buffer: 100% Nonidet P-40,…

A: Molar concentration, also known as molarity, it is a number of moles of solute in a solution. Molar…

Q: Calculate the cocentrations of acetic acid (pKa = 4.76) and sodium acetate necessary to prepare a…

A:

Q: If an aqueous solution has a hydroxyl ion concentration of 1 x 10-6 M, what is the concentration of…

A: Water dissociates into hydrogen ions and hydroxyl ions and at neutral pH (pH =7) the numbers of…

Q: pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054…

A: We can do this by multiplying the concentration in the undiluted supernatant by the dilution factor…

Q: 1) A solution is made by combining 128 mL of ethanol and 0.452 L of water. In this solution, what is…

A: Hi! Thanks for your question. But as you have posted multiple questions, I am answering only the…

Q: 50 mL of 5mM NaCl solution from 1 M NaCl solution

A: Dilution is the process by which we decrease the concentration of a solution. This process is vital…

Q: You have 150 g of dextrose available and you need to compound a 20% solution. If you use the entire…

A: When we describe a concentration as a percentage without specifying the type of formula, we imply…

Q: Aspirin (acetylsalicylic acid, structure below), has a pKa of 3.5. The pH at which aspirin is most…

A: Therefore, aspirin is most soluble in water when the pH is significantly higher than its pKa (pH…

Q: The calibration curve shown below was used to analyze an unknown protein solution. What is the…

A: The unknown concentration of a substance in the given sample can be estimated by comparing the…

Q: ratio [CH3COOH]/[N2CH3COO]

A: The dissociation equation for CH3COOH is: CH3COOH <-----> CH3COO- + H+ So the expression for…

Q: You need to make a protein buffer of: 100 mM NaCl ⚫ 25 mM Tris 8 • • 5% w/v glycerol 2 mM DTT Your…

A: In order to calculate how much of each component we should use to get the required buffer, we need…

Q: 3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following…

A: Calculations for a substrate solution's dilution are related to the subject addressed above. A…

Q: 200 microliters of a standard solution of 0.200 mg/mL caffeine was mixed with 4.8 mL of 50 mM sodium…

A: Equation of dilution: M1 × V1 = M2 × V2where:M1 is the molar concentration of the stock solution.M2…

Q: Calculate the pH of a buffer that contains 0.15 M MOPS anionic form and 0.25M MOPS zwitterion.  The…

A: The zwitterion is the form of amino acid which has net charge of zero. The Henderson-Hasselbalch…

Q: Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.

A: A buffer is defined as the mixture of a weak acid and its conjugate base or weak base and its…

Q: You need to make 20 mL of a 1/100 (10-2) dilution of a crystal violet solution? How much diluting…

A: Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline…

Q: To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul  100% NP-40 solution is…

A: Data given is, C1 = 100, C2 = 0.3, V2 = 100ML, V1 = ? C1= Initial concentration;            C2 =…

Q: A 0.200 M solution of a weak monoprotic acid (HA) has a pH of 2.35. What is the value of K of this…

A: The final answers are: Ka​=9.977∗10−5Percent ionization =2.2335% Explanation:Step 1:The equation is…

Q: Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of…

A: Sedimentation Coefficient is the rate per unit centrifugal field experienced by the particle…

Q: To prepare 500 mL of a 1X TAE buffer solution, we need to mix solution (so 25 times more…

A: TAE is a standard buffer for the preparation and operation of DNA agarose gels. TBE provides…

Q: pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054…

A: To calculate the amount of soluble protein (in grams) in the soy flour extract, we need to use the…

Q: Protein Solubility pll 3.5 4.5 6.5 7.5 Dilution Factor 5 5 50 50 Absorbance 0.098 0.027 0.028 0.032…

A: Protein solubility is defined as the concentration of protein in a saturated solution that is in…

Q: What is the final concentration if 239mL of a 3.9M glucose solution is diluted to a volume of 842mL?

A: To solve this we will use the formula - M1xV1= M2xV2

Q: You need to prepare 1L of a 5×TE stock solution (1×TE buffer:10mM Tris,1mM EDTA).How much of Tris…

A: The mole is used as a unit in the SI system to quantify the amount of substance present. One mole is…

Q: Show how to make up 500ml of 0.5M Tris-HCl at pH 8.8

A: Tris-HCl used in buffer preparation to perform various chemical reactions.

Q: Thimerosal Tincture USP contains 0.1% w/v thimerosal and 50% v/v ethyl alcohol. If the cap is left…

A: The objective of this question is to determine the concentration of thimerosal in the evaporated…

Q: Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength…

A: TAE buffer stands for Tris-acetate-EDTA buffer. It is used in lab to do electrophoresis of nucleic…

Q: intensity €495 1295 567.5 567.9 1995 s

A: M/z means mass to charge ratio . The first step is involves selecting largest leaks in mass spectrum…

Q: What is the pH of a solution of 100 ml of 0.01 M H3PO4 and 100 ml of 0.01 M Na3PO4?

A: A buffer is an aqueous solution containing a weak acid and its conjugate base or a weak base and its…

Q: PK 12.5 HO. ОН PK,43 Compound X О

A: The compound X has two distinct stages, corrresponding to deprotonation of two different groups. At…

Q: You are preparing 1 Lt of TAE (Tris Acetate EDTA) gel running buffer from a 50x stock solution. How…

A: In a gel running buffer 1x TAE buffer is used. Given, Final volume = 1 liter Stock solution is 50x…

Q: moll' What is the pH of this solution? What is the pH of 35 ml buffer solution which is 0,3 M in…

A: Given Values: Volume of the buffer = 35 ml Concentration of acetic acid = 0.3 M Concentration of…

Q: How would I calculate the amount of each component in each solution?  Stock solutions provided:…

A: In-order to perform dilution, we take a specific volume from stock solution and transfer it to…

Q: It takes 175ml of 0.75 M H2SO4   to completely neutralize 285 ml of NaOH.  What is the concentration…

A: Neutralization is a chemical reaction in which acid and a base react quantitatively with each other.