Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
200 microliters of a standard solution of 0.200 mg/mL caffeine was mixed with 4.8 mL of 50 mM sodium phosphate buffer, pH 3: methanol (60:40 v/v). What is the concentration of caffeine, in mg/mL, in this solution? Give your answer to two significant figures.

200 microliters of a standard solution of 0.200 mg/mL caffeine was mixed with 4.8 mL of 50 mM sodium phosphate buffer, pH 3: methanol (60:40 v/v). What is the concentration of caffeine, in mg/mL, in this solution? Give your answer to two significant figures.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
**Problem Statement:** 200 microliters of a standard solution of 0.200 mg/mL caffeine was mixed with 4.8 mL of 50 mM sodium phosphate buffer, pH 3: methanol (60:40 v/v). What is the concentration of caffeine, in mg/mL, in this solution? Give your answer to two significant figures. **Solution:** This problem requires the calculation of the concentration of caffeine in a mixed solution. Here is how to solve it step-by-step. 1. **Calculate the Total Volume of the Solution:** - Volume of caffeine solution = 200 microliters = 0.200 mL - Volume of buffer solution = 4.8 mL - Total volume = 0.200 mL + 4.8 mL = 5.0 mL 2. **Calculate the Amount of Caffeine:** - Concentration of caffeine solution = 0.200 mg/mL - Amount of caffeine = concentration × volume = 0.200 mg/mL × 0.200 mL = 0.040 mg 3. **Calculate the New Concentration of Caffeine in the Mixed Solution:** - New concentration = amount of caffeine / total volume - New concentration = 0.040 mg / 5.0 mL = 0.0080 mg/mL Thus, the concentration of caffeine in the mixed solution is **0.0080 mg/mL** (to two significant figures).
Transcribed Image Text:**Problem Statement:** 200 microliters of a standard solution of 0.200 mg/mL caffeine was mixed with 4.8 mL of 50 mM sodium phosphate buffer, pH 3: methanol (60:40 v/v). What is the concentration of caffeine, in mg/mL, in this solution? Give your answer to two significant figures. **Solution:** This problem requires the calculation of the concentration of caffeine in a mixed solution. Here is how to solve it step-by-step. 1. **Calculate the Total Volume of the Solution:** - Volume of caffeine solution = 200 microliters = 0.200 mL - Volume of buffer solution = 4.8 mL - Total volume = 0.200 mL + 4.8 mL = 5.0 mL 2. **Calculate the Amount of Caffeine:** - Concentration of caffeine solution = 0.200 mg/mL - Amount of caffeine = concentration × volume = 0.200 mg/mL × 0.200 mL = 0.040 mg 3. **Calculate the New Concentration of Caffeine in the Mixed Solution:** - New concentration = amount of caffeine / total volume - New concentration = 0.040 mg / 5.0 mL = 0.0080 mg/mL Thus, the concentration of caffeine in the mixed solution is **0.0080 mg/mL** (to two significant figures).
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS