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Given: Beer-Lambert Law A = Ecl E = 8000 for Met myoglobin E= 14,000 for oxy-myoglobin l=1 cm find c, the concentration of Oxy-Mb and Met-Mb based on the data table given below.

Given: Beer-Lambert Law A = Ecl E = 8000 for Met myoglobin E= 14,000 for oxy-myoglobin l=1 cm find c, the concentration of Oxy-Mb and Met-Mb based on the data table given below.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Given: Beer-Lambert Law A = Ecl

E = 8000 for Met myoglobin

E= 14,000 for oxy-myoglobin

l=1 cm

find c, the concentration of Oxy-Mb and Met-Mb based on the data table given below.

 

**Absorption Table:** This table presents the absorbance values of two forms of myoglobin: Met-Myoglobin (Fe3+) and Oxy-Myoglobin (Fe2+), under different conditions. Absorbance is a measure of the amount of light absorbed by a sample. | Condition | Met-Myoglobin (Fe3+) | Oxy-Myoglobin (Fe2+) | |-------------------------|----------------------|----------------------| | Original | 0.270 | 0.400 | | Oxidized Sample Fe3+ | 0.760 | 0.120 | | Reduced Sample Fe2+ | 0.100 | 0.783 | **Key Points:** - **Original Condition:** Refers to the initial state of the samples. Here, Met-Myoglobin shows an absorbance of 0.270, while Oxy-Myoglobin shows 0.400. - **Oxidized Sample Fe3+:** This condition increases the absorbance of Met-Myoglobin to 0.760 and decreases that of Oxy-Myoglobin to 0.120, indicating significant oxidation. - **Reduced Sample Fe2+:** In the reduced state, Met-Myoglobin has a lower absorbance of 0.100, and Oxy-Myoglobin shows a high absorbance of 0.783, indicating reduction. The absorbance was calculated using the Beer-Lambert Law, where \( A = \epsilon c l \). Here, \( A \) is the absorbance (unitless), \( \epsilon \) is the molar absorptivity, \( c \) is the concentration, and \( l \) is the path length. Note that absorbance values do not have units.
Transcribed Image Text:**Absorption Table:** This table presents the absorbance values of two forms of myoglobin: Met-Myoglobin (Fe3+) and Oxy-Myoglobin (Fe2+), under different conditions. Absorbance is a measure of the amount of light absorbed by a sample. | Condition | Met-Myoglobin (Fe3+) | Oxy-Myoglobin (Fe2+) | |-------------------------|----------------------|----------------------| | Original | 0.270 | 0.400 | | Oxidized Sample Fe3+ | 0.760 | 0.120 | | Reduced Sample Fe2+ | 0.100 | 0.783 | **Key Points:** - **Original Condition:** Refers to the initial state of the samples. Here, Met-Myoglobin shows an absorbance of 0.270, while Oxy-Myoglobin shows 0.400. - **Oxidized Sample Fe3+:** This condition increases the absorbance of Met-Myoglobin to 0.760 and decreases that of Oxy-Myoglobin to 0.120, indicating significant oxidation. - **Reduced Sample Fe2+:** In the reduced state, Met-Myoglobin has a lower absorbance of 0.100, and Oxy-Myoglobin shows a high absorbance of 0.783, indicating reduction. The absorbance was calculated using the Beer-Lambert Law, where \( A = \epsilon c l \). Here, \( A \) is the absorbance (unitless), \( \epsilon \) is the molar absorptivity, \( c \) is the concentration, and \( l \) is the path length. Note that absorbance values do not have units.
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