53A%252F%252FIMS.mheducation.com%252FmghmicsignmentSaved2 attempts leftCheck my workEnter your answer in the provided box.Given two complementary strands of DNA containing 1.08 x 102 base pairs each, calculate the ratio oftwo separate strands to hydrogen-bonded double helix in solution at 3.32 x 102 K. (Hint: The formula forcalculating this ratio is e AE/R", where AE is the energy difference between hydrogen-bonded double-strand DNAS and single-strand DNAS and R is the gas constant.) Assume the energy of hydrogen bondsper base pair to be 7.70 x 10 kJ/mol under laboratory conditions. This model is a simplification ofreality.Guide

A double stranded DNA is formed as 2 single strands of DNA pair with each other via hydrogen…

Answered: Given two complementary strands of DNA…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The figure shows that the average distance between base pairs measured parallel to the axis of a DNA molecule is 3.4 Å. The average molar mass of a pair of nucleotides is 650 g•mol-1. Estimate the length in cm of a DNA molecule of molar mass 5.1x109 4.0 .26 g•mol-1. cm Roughly how many base pairs are contained in this molecule? [4.0 784615 x base pairs CG G C OH TA Thymine Adenine H. H. OH CH2 N-H.......O CH3 H H N……·H–N HLH TA OH 0-P H2C CG G C H. CH2 Guanine H OH Cytosine ... H-N TA AT H' N.....H-N HLH N-H.......O H2C OH H -o. -P3D0 TA 0- OH H. -CH2 (b) The most common structure of DNA, which is a right-handed double helix. The two strands are held together by hydrogen bonds (a) Base-pair formation between adenine (A) and thymine (T) and between cytosine (C) and guanine (G). and other intermolecular forces.
Each group will be provided with 20 g double-stranded DNA oligomer in STE buffer (0.1M NaCl/ Tris/ 10 mM EDTA, pH 7.4). The sequence of one strand of the oligomer is: 5’-AATGGTACGTATACTTTAT-3’ - show a table of % denaturation of DNA at different temperatures, using the equation provided in “Calculation” - plot a graph of % denaturation of DNA vs different temperatures with clear label and title, and hence determine the experimental Tm of the DNA sample - calculate the theoretical Tm of the sample using Marmur and Doty equation, compare the experimental Tm with theoretical Tm of the DNA sample
Draw atleast 3 structure of nucleotide containing 2 pyrimidine bases and I purine base.
Compare the melting temperature of a 1-kb segment of DNA containing20% A residues to that of a 1-kb segment containing 30% A residues under the same conditions.
CAN YOU PLEASE ANSWER 1b 1a) The phosphate groups in the sugar-phosphate backbone of each strand of a DNA molecule have a pKa of ~2.14. Normal physiological pH in most cells is 7.0-7.5, which is significantly above the pKa for the phosphate groups, so every phosphate group in the backbone of both strands of a DNA molecule is deprotonated and carries a -1 charge - but DNA in cells is normally double-stranded. Briefly explain, in your own words, why the large negative charges on the backbones of the two strands of a double-stranded DNA molecule don't cause the strands to separate under normal cellular conditions. 1b) If you raise the pH to 12, but keep everything else about the solution in which the double-stranded DNA is dissolved the same, the two strands of DNA will separate/the DNA will denature. Briefly explain, in your own words, why this happens - why does DNA denature at pH 12 but not at pH 7?
The folding and unfolding rate constants for a myoglobin mutant have been determined. The unfolding rate constant ke-u = 3.62 x 10-55 and the folding rate constant ku-p = 255 s1, where Fis the folded protein and U is the unfolded (denatured) protein. For wild-type myoglobin, AG;u = +37.4 kJ/mol. Which myoglobin is more thermodynamically stable, the mutant or the wild-type?
Given the following eukaryotic DNA strand, transcribe and translate the DNA into a polypeptide using the 3’ – 5’ strand as the template. You may use drawings, diagrams, colours and annotations to describe how the DNA strand will be synthesized into a functional protein. (KEY: The letters SBMD are “made up” nucleic acids that depict non-coding regions in the DNA, hypothetically S pairs with B and M pairs with D).2.2. Describe what are missense mutations and its effects on structure and function using haemoglobin as an example
A primitive eukaryote was discovered that displayed a uniquenucleic acid as its genetic material. Analysis revealed the followingobservations:(a) X-ray diffraction studies display a general pattern similarto DNA, but with somewhat different dimensions and moreirregularity. (b) A major hyperchromic shift is evident upon heating andmonitoring UV absorption at 260 nm.(c) Base-composition analysis reveals four bases in the followingproportions:Adenine = 8% Hypoxanthine = 18%Guanine = 37% Xanthine = 37%(d) About 75 percent of the sugars are deoxyribose, whereas25 percent are ribose.Attempt to solve the structure of this molecule by postulating amodel that is consistent with the foregoing observations.
Briefly explain why a "Folding Funnel" is used to represent the kinetics of folding (limit 5-6 sentences)?
a and b please
HN N C N H₂N HN پرسپولیو D B CH₂ -N N NH₂ E When part of a nucleotide in a nucleic acid chain, which of the following may base pair with thymine nucleotides? Choose all correct answers and assume normal Watson-Crick base pairing.

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