For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials (Table 1). Please note that the final reaction volume differs between the three setups. Vial No. A (ml) В (ml) C (ml) H20 1 5 Tris/HCl buffer pH7|1 1 1 Tumour cells (5% 1 4 (w/v)) in buffer Glucose [25mM] 0.5 0.75 1 Table 1. a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings. b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings. Based on Table 1, calculate the glucose concentration / 1 gram tumour cells in conditions A, B and C. Show your workings. 3.

For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials (Table 1). Please note that the final reaction volume differs between the three setups. Vial No. A (ml) В (ml) C (ml) H20 1 5 Tris/HCl buffer pH7|1 1 1 Tumour cells (5% 1 4 (w/v)) in buffer Glucose [25mM] 0.5 0.75 1 Table 1. a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings. b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings. Based on Table 1, calculate the glucose concentration / 1 gram tumour cells in conditions A, B and C. Show your workings. 3.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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